BTF Recombinant Rabbit Monoclonal Antibody [JE77-57] (HA722374)
Catalog# HA722374
Rabbit Monoclonal to BTF
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of BTF on different lysates with Rabbit anti-BTF antibody (HA722374) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C6 cell lysate
Lane 5: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 106 kDa
Observed band size: 140 kDa
Exposure time: Lane 1-2: 20 seconds; Lane 3-5: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722374) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: C6 cell lysate
Lane 5: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 106 kDa
Observed band size: 140 kDa
Exposure time: Lane 1-2: 20 seconds; Lane 3-5: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722374) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.