Breast cancer-overexpressed gene 1 protein Mouse Monoclonal Antibody [

Western blot analysis on recombinant protein using anti-KIAA0100 Mouse mAb (Cat. # M1210-6).

Applications

WB
ICC
IHC-P

REACTIVITY

Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Breast cancer-overexpressed gene 1 protein Mouse Monoclonal Antibody [A0-F3] (M1210-6)

Immunogen

Recombinant protein within human kiaa0100 1900-2250 aa.

Host

Mouse

Positive Control

Hela, human breast carcinoma tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A0-F3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

254kDa

Isotype

IgG1

APPLICATION DILUTION

WB
1:1,000-1:10,000

ICC
1:100-1:500

IHC-P
1:200

TARGET

UNIPROT #

Q14667

PROTEIN NAME

Breast cancer-overexpressed gene 1 protein

SYNONYMS

Antigen MLAA-22 antibody; BCOX antibody; BCOX1 antibody; Breast cancer-overexpressed gene 1 protein antibody; CT101 antibody; DKFZp686M0843 antibody; K0100_HUMAN antibody; KIAA0100 antibody; MGC111488 antibody; MGC134981 antibody; UPF0378 protein KIAA0100 antibody

SEQUENCE SIMILARITIES

Belongs to the SABRE family.

TISSUE SPECIFICITY

Expressed in pancreas, placenta and up-regulated in breast carcinoma epithelial cells, ductal in situ carcinoma (DCIS), invasive breast carcinoma (IBC) and metastatic breast carcinoma cells (MET).

SUBCELLULAR LOCATION

secreted

FUNCTION

Breast cancer-overexpressed gene 1 protein（KIAA0100）is a glucoprotein composed of 2235 amino acids.KIAA0100 has four isforms (254kDa,250kDa,45kDa,25kDa).KIAA0100 is expressed in pancreas, placenta and up-regulated in breast carcinoma epithelial cells.

Goat IgG Light chain Mouse Monoclonal Antibody [B8-C4-G4] (M0910-2)

Applications

WB
ELISA
IP

REACTIVITY

Goat

Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Thymidine phosphorylase Mouse Monoclonal Antibody [A1A8] (HA600036)

Applications

REACTIVITY

Human

Western blot analysis of Thymidine phosphorylase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600036, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

MMAF Mouse Monoclonal Antibody [A5G11] (HA600035)

Applications

ELISA

REACTIVITY

The binding activity of HA600035 with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600035) for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600035) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

MMAF Mouse Monoclonal Antibody [A5G12] (HA600034)

Applications

ELISA

REACTIVITY

The binding activity of (HA600034) with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody () for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600034) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

IGF1R Mouse Monoclonal Antibody [D5-G2] (HA600033)

Applications

REACTIVITY

Human

Western blot analysis of IGF1R on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)

Applications

WB
IHC-P

REACTIVITY

Human
Mouse
Rat