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PRODUCT CODE: ET7109-07

BRD2 Recombinant Rabbit Monoclonal Antibody [JG40-42] (ET7109-07)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1/2,000 dilution.
  • Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1/2,000 dilution.
  • ICC staining BRD2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining BRD2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining BRD2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRD2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BRD2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1/2,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

BRD2 Recombinant Rabbit Monoclonal Antibody [JG40-42] (ET7109-07)

Immunogen

Recombinant protein within human brd2 aa 1-200.

Host

Rabbit

Positive Control

SiHa, 293T, A431, human tonsil tissue, human colon tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG40-42

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

Predicted band size 88 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P25440

PROTEIN NAME

BRD2

SYNONYMS

BRD 2 antibody; Brd2 antibody; BRD2_HUMAN antibody; Bromodomain containing 2 antibody; Bromodomain-containing protein 2 antibody; D6S113E antibody; DKFZp686N0336 antibody; Female sterile homeotic related gene 1 antibody; Female sterile homeotic related gene 1, mouse, homolog of antibody; FLJ31942 antibody; FSH antibody; FSRG1 antibody; KIAA9001 antibody; NAT antibody; O27.1.1 antibody; Really interesting new gene 3 protein antibody; RING3 antibody; RING3 PROTEIN antibody; RNF3 antibody

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The bromodomain-containing proteins include BRD2, BRD3, BRD4 and BRDT. BRD2 (RING3 protein) is a mitogen-activated nuclear protein whose gene is located in the human MHC II region, suggesting its relation to HLA-associated diseases. The gene encoding BRD3 (RING3-like protein) contains two bromodomains and maps to chromosome 9q34. BRD4 (HUNK1 protein) is a nuclear protein involved in the regulation of chromosomal dynamics during mitosis. The testis-specific bromodomain protein BRDT contains a PEST sequence, indicating that it undergoes rapid intracellular degradation. The bromodomain-containing proteins are ubiquitously expressed.