PRODUCT CODE: ET1611-6

BPI Recombinant Rabbit Monoclonal Antibody [SN06-63] (ET1611-6)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Zebrafish

Western blot analysis of BPI on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of BPI on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of BPI was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-6, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of BPI on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

BPI Recombinant Rabbit Monoclonal Antibody [SN06-63] (ET1611-6)

Immunogen

Synthetic peptide within human bpi aa 120-170.

Host

Rabbit

Positive Control

THP-1.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN06-63

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

57 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

BPI

SYNONYMS

Bactericidal permeability-increasing protein antibody; bactericidal/permeability-increasing protein antibody; BPI antibody; BPI fold containing family D, member 1 antibody; BPIFD1 antibody; CAP 57 antibody; rBPI antibody; recombinant BPI holoprotein, rBPI antibody

SEQUENCE SIMILARITIES

Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

TISSUE SPECIFICITY

Restricted to cells of the myeloid series.

SUBCELLULAR LOCATION

Secreted, Cytoplasmic granule membrane.

FUNCTION

The bactericidal permeability increasing protein (BPI) is an antibacterial and endotoxin-neutralizing molecule that is abundant in the granules of polymorphonuclear leukocytes (neutrophil granules). The 31.5-kb-long human BPI gene maps to chromosome 20q11.23-q12, contains 15 exons, and encodes a 456 amino acid protein. Epithelial cells which line mucosal surfaces are the first line of defense against bacterial invasion and infection. BPI localizes to the cell surface of epithelial cells and blocks endotoxin-mediated signaling, thereby protecting mucosal surfaces against gram-negative bacteria and their endotoxin. BPI, lipopolysaccharide binding protein (LBP), phospholipid transfer protein (PLTP), and cholesteryl ester transfer protein (CETP) constitutes a family of functionally related proteins.