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PRODUCT CODE: ER1803-97

BOB1 Rabbit Polyclonal Antibody (ER1803-97)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of BOB1 on Daudi lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of BOB1 on Daudi lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of BOB1 was done on Daudi cells. The cells were fixed, permeabilized and stained with BOB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Western blot analysis of BOB1 on Daudi lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

BOB1 Rabbit Polyclonal Antibody (ER1803-97)

Immunogen

Recombinant protein within human bob1 aa 10-180.

Host

Rabbit

Positive Control

Daudi.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

35 kDa (predicted molecular weight: 27 kDa)

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500

  • FC:1:50-1:100

TARGET

UNIPROT #

Q16633

PROTEIN NAME

BOB1

SYNONYMS

B cell Oct binding protein 1 antibody; B cell specific coactivator OBF 1 antibody; B cell specific coactivator OBF1 antibody; B-cell-specific coactivator OBF-1 antibody; BOB 1 antibody; BOB-1 antibody; OBF 1 antibody; OBF1 antibody; OBF1_HUMAN antibody; OCA B antibody; OCA-B antibody; OCAB antibody; OCT binding factor 1 antibody; OCT-binding factor 1 antibody; POU class 2 associating factor 1 antibody; POU domain class 2 associating factor 1 antibody; POU domain class 2-associating factor 1 antibody; Pou2af1 antibody

SEQUENCE SIMILARITIES

Belongs to the POU2AF1 family.

TISSUE SPECIFICITY

B-cell specific.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Reduced activation of Bob1-deficient B cells following antigenic stimulation is often reported. The action of Bob1 on B cell maturation and tolerance could be mediated by effects on B cell activation, in part by regulating the expression of immunosuppressive miRNAs. Bob1 has long been considered a B cell‐specific factor that interacts with the transcription factors Oct1 and Oct2 to enhance octamer‐dependent transcription. Bob1 can directly bind to and transactivate the promoters of Bcl6 and Btla.