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PRODUCT CODE: ER1802-66

BNIP1 Rabbit Polyclonal Antibody (ER1802-66)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Rat

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Western blot analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution.
  • Western blot analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution.
  • ICC staining BNIP1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining BNIP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-BNIP1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-BNIP1 antibody. Counter stained with hematoxylin.
Western blot analysis of BNIP1 on rat liver tissue lysate using anti-BNIP1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

BNIP1 Rabbit Polyclonal Antibody (ER1802-66)

Immunogen

Recombinant protein within human bn1p1 aa 27-228 / 228.

Host

Rabbit

Positive Control

Rat liver tissue lysate, HepG2, LOVO, human liver tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

26 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:100

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

TARGET

UNIPROT #

Q12981

PROTEIN NAME

BNIP1

SYNONYMS

BCL2 adenovirus E1B 19kD interacting protein 1 antibody; BCL2/adenovirus E1B 19 kDa protein-interacting protein 1 antibody; BNIP1 antibody; MGC41600 antibody; Nip1 antibody; OTTHUMP00000161077 antibody; OTTHUMP00000161078 antibody; OTTHUMP00000161079 antibody; OTTHUMP00000223580 antibody; sec20 antibody; SEC20_HUMAN antibody; SEC20L antibody; Transformation-related gene 8 protein antibody; TRG-8 antibody; TRG8 antibody; Vesicle transport protein SEC20 antibody

SEQUENCE SIMILARITIES

Belongs to the SEC20 family.

TISSUE SPECIFICITY

Isoform 1 is highly expressed in heart, brain, liver skeletal muscle and pancreas. Isoform 3 is moderately expressed in placenta, lung and kidney. Isoform 4 is highly expressed in testis and small intestine.

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated. 'Lys-63'-linked polyubiquitination by RNF185 increases the interaction with the autophagy receptor SQSTM1. Undergoes 'Lys-29'- and 'Lys-63'-linked polyubiquitination by RNF186 that may regulate BNIP1 localization to the mitochondrion.

SUBCELLULAR LOCATION

Endoplasmic reticulum. Mitochondrion.

FUNCTION

The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria.