PRODUCT CODE: EM1701-55

Bmi1 Mouse Monoclonal Antibody [A11-C11] (EM1701-55)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Bmi1 on Hela (1) and NIH-3T3 (2) using anti-Bmi1 antibody at 1/500 dilution.
  • Western blot analysis of Bmi1 on Hela (1) and NIH-3T3 (2) using anti-Bmi1 antibody at 1/500 dilution.
  • ICC staining Bmi1 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
Western blot analysis of Bmi1 on Hela (1) and NIH-3T3 (2) using anti-Bmi1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Bmi1 Mouse Monoclonal Antibody [A11-C11] (EM1701-55)

Immunogen

Recombinant protein within mouse bmi1 aa 1-324 / 324.

Host

Mouse

Positive Control

Hela, NIH-3T3, SH-SY-5Y, human colon cancer tissue, mouse spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A11-C11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein affinity purified

MOLECULAR WEIGHT

42 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200-1:400

TARGET

UNIPROT #

PROTEIN NAME

Bmi1

SYNONYMS

B lymphoma Mo MLV insertion region (mouse) antibody; B lymphoma Mo MLV insertion region 1 homolog antibody; Bmi 1 antibody; BMI1 antibody; BMI1 polycomb ring finger oncogene antibody; BMI1_HUMAN antibody; Flvi 2/bmi 1 antibody; FLVI2/BMI1 antibody; MGC12685 antibody; Murine leukemia viral (bmi 1) oncogene homolog antibody; Oncogene BMI 1 antibody; PCGF 4 antibody; PCGF4 antibody; Polycomb complex protein BMI 1 antibody; Polycomb complex protein BMI-1 antibody; Polycomb group protein Bmi1 antibody; Polycomb group ring finger 4 antibody; Polycomb group RING finger protein 4 antibody; RING finger protein 51 antibody; RNF 51 antibody; RNF51 antibody

POST-TRANSLATIONAL MODIFICATION

Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

In Drosophila, Polycomb (Pc-g) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. Mel-18 and Bmi-1, mammalian homologs of Drosophila Pc-g group proteins, are similarly expressed during development and implicated in the regulation of gene expression, axial skeleton development, control of proliferation and survival of haematopoietic cells. Mel-18 directly binds to DNA through a RING-finger motif and preferentially associates with juxtaposed enhancer elements on various genes, including Bcl-2, c-Myc and Hox. Mel-18 is an immediate early response gene within the c-Myc/Cdc25 signaling cascade that exhibits tumor suppressor activity and negatively regulates cell cycle progression by blocking S phase entry. Alternatively, Bmi-1 has been identified as a potent oncogene as it contributes to the transcriptional activation of genes implicated in early lymphoid development. Proviral activation of Bmi-1 expression corresponds to enhanced gene-specific activation of other proto-oncogenes, including c-Myc and Pim, subsequently resulting in the progression of lymphomagenesis.