PRODUCT CODE: ER1706-71

BMAL1 Rabbit Polyclonal Antibody (ER1706-71)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of BMAL1 on SYSH5Y cell lysates using anti-BMAL1 antibody at 1/1,000 dilution.
  • Western blot analysis of BMAL1 on SYSH5Y cell lysates using anti-BMAL1 antibody at 1/1,000 dilution.
  • ICC staining BMAL1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining BMAL1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cncer tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-BMAL1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SHSY5Y cells with BMAL1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of BMAL1 on SYSH5Y cell lysates using anti-BMAL1 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

BMAL1 Rabbit Polyclonal Antibody (ER1706-71)

Immunogen

Recombinant protein within n-terminal human bmal1.

Host

Rabbit

Positive Control

SYSH5Y, A549, MCF-7, human liver cancer tissue, human prostate tissue, mouse brain tissue, mouse prostate tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

68 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

BMAL1

SYNONYMS

ARNT like protein 1 brain and muscle antibody; Arntl antibody; Aryl hydrocarbon receptor nuclear translocator like antibody; Aryl hydrocarbon receptor nuclear translocator like protein 1 antibody; Aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody; Basic helix loop helix PAS orphan MOP3 antibody; Basic helix loop helix PAS protein MOP3 antibody; Basic-helix-loop-helix-PAS protein MOP3 antibody; bHLH PAS protein JAP3 antibody; bHLH-PAS protein JAP3 antibody; bHLHe5 antibody; BMAL 1 antibody; BMAL1_HUMAN antibody; BMAL1c antibody; Brain and muscle ARNT like 1 antibody; Brain and muscle ARNT-like 1 antibody; CG8727 PA antibody; Class E basic helix-loop-helix protein 5 antibody; cycle antibody; JAP 3 antibody; JAP3 antibody; Member of PAS protein 3 antibody; Member of PAS superfamily 3 antibody; MGC47515 antibody; MOP 3 antibody; MOP3 antibody; PAS domain-containing protein 3 antibody; PASD 3 antibody; PASD3 antibody; TIC antibody

TISSUE SPECIFICITY

Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP9X.; O-glycosylated; contains O-GlcNAc. O-glycosylation by OGT prevents protein degradation by inhibiting ubiquitination. It also stabilizes the CLOCK-ARNTL/BMAL1 heterodimer thereby increasing CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2/3 and CRY1/2.; Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression. Deacetylated by SIRT1, which may result in decreased protein stability.; Phosphorylated upon dimerization with CLOCK. Phosphorylation enhances the transcriptional activity, alters the subcellular localization and decreases the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. Phosphorylation shows circadian variations in the liver with a peak between CT10 to CT14. Phosphorylation at Ser-90 by CK2 is essential for its nuclear localization, its interaction with CLOCK and controls CLOCK nuclear entry (By similarity). Dephosphorylation at Ser-78 is important for dimerization with CLOCK and transcriptional activity.; Sumoylated on Lys-259 upon dimerization with CLOCK. Predominantly conjugated to poly-SUMO2/3 rather than SUMO1 and the level of these conjugates undergo rhythmic variation, peaking at CT9-CT12. Sumoylation localizes it exclusively to the PML body and promotes its ubiquitination in the PML body, ubiquitin-dependent proteasomal degradation and the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer.; Undergoes lysosome-mediated degradation in a time-dependent manner in the liver.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

BMAL1, ARNTL2 (aryl hydrocarbon receptor nuclear translocator-like 2) is a basic Helix-Loop-Helix-Per-Arnt-Sim (bHLH-PAS) transcription factor. ARNTL2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription. Also, in umbilical vein endothelial cells, ARNTL2 activates SERPINE1 through E-box sites. This transactivation is inhibited by PER2 and CRY1. The NPAS2-ARNTL/BMAL1 heterodimer positively regulates the expression of MAOA, F7 and LDHA and modulates the circadian rhythm of daytime contrast sensitivity by regulating the rhythmic expression of adenylate cyclase type 1 (ADCY1) in the retina. The CLOCK-ARNTL/BMAL1 heterodimer also recognizes the non-canonical E-box motifs 5'-AACGTGA-3' and 5'-CATGTGA-3'. Essential for the rhythmic interaction of CLOCK with ASS1 and plays a critical role in positively regulating CLOCK-mediated acetylation of ASS1.