Rabbit polyclonal primary
BMAL1 Rabbit Polyclonal Antibody (ER1706-71)
Recombinant protein within n-terminal human bmal1.
SYSH5Y, A549, MCF-7, human liver cancer tissue, human prostate tissue, mouse brain tissue, mouse prostate tissue, rat brain tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Protein affinity purified.
ARNT like protein 1 brain and muscle antibody; Arntl antibody; Aryl hydrocarbon receptor nuclear translocator like antibody; Aryl hydrocarbon receptor nuclear translocator like protein 1 antibody; Aryl hydrocarbon receptor nuclear translocator-like protein 1 antibody; Basic helix loop helix PAS orphan MOP3 antibody; Basic helix loop helix PAS protein MOP3 antibody; Basic-helix-loop-helix-PAS protein MOP3 antibody; bHLH PAS protein JAP3 antibody; bHLH-PAS protein JAP3 antibody; bHLHe5 antibody; BMAL 1 antibody; BMAL1_HUMAN antibody; BMAL1c antibody; Brain and muscle ARNT like 1 antibody; Brain and muscle ARNT-like 1 antibody; CG8727 PA antibody; Class E basic helix-loop-helix protein 5 antibody; cycle antibody; JAP 3 antibody; JAP3 antibody; Member of PAS protein 3 antibody; Member of PAS superfamily 3 antibody; MGC47515 antibody; MOP 3 antibody; MOP3 antibody; PAS domain-containing protein 3 antibody; PASD 3 antibody; PASD3 antibody; TIC antibody
Hair follicles (at protein level). Highly expressed in the adult brain, skeletal muscle and heart.
Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP9X.; O-glycosylated; contains O-GlcNAc. O-glycosylation by OGT prevents protein degradation by inhibiting ubiquitination. It also stabilizes the CLOCK-ARNTL/BMAL1 heterodimer thereby increasing CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2/3 and CRY1/2.; Acetylated on Lys-538 upon dimerization with CLOCK. Acetylation facilitates CRY1-mediated repression. Deacetylated by SIRT1, which may result in decreased protein stability.; Phosphorylated upon dimerization with CLOCK. Phosphorylation enhances the transcriptional activity, alters the subcellular localization and decreases the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. Phosphorylation shows circadian variations in the liver with a peak between CT10 to CT14. Phosphorylation at Ser-90 by CK2 is essential for its nuclear localization, its interaction with CLOCK and controls CLOCK nuclear entry (By similarity). Dephosphorylation at Ser-78 is important for dimerization with CLOCK and transcriptional activity.; Sumoylated on Lys-259 upon dimerization with CLOCK. Predominantly conjugated to poly-SUMO2/3 rather than SUMO1 and the level of these conjugates undergo rhythmic variation, peaking at CT9-CT12. Sumoylation localizes it exclusively to the PML body and promotes its ubiquitination in the PML body, ubiquitin-dependent proteasomal degradation and the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer.; Undergoes lysosome-mediated degradation in a time-dependent manner in the liver.
BMAL1, ARNTL2 (aryl hydrocarbon receptor nuclear translocator-like 2) is a basic Helix-Loop-Helix-Per-Arnt-Sim (bHLH-PAS) transcription factor. ARNTL2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription. Also, in umbilical vein endothelial cells, ARNTL2 activates SERPINE1 through E-box sites. This transactivation is inhibited by PER2 and CRY1. The NPAS2-ARNTL/BMAL1 heterodimer positively regulates the expression of MAOA, F7 and LDHA and modulates the circadian rhythm of daytime contrast sensitivity by regulating the rhythmic expression of adenylate cyclase type 1 (ADCY1) in the retina. The CLOCK-ARNTL/BMAL1 heterodimer also recognizes the non-canonical E-box motifs 5'-AACGTGA-3' and 5'-CATGTGA-3'. Essential for the rhythmic interaction of CLOCK with ASS1 and plays a critical role in positively regulating CLOCK-mediated acetylation of ASS1.