PRODUCT CODE: M1701-10

BLCAP Mouse Monoclonal Antibody [4E1] (M1701-10)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Rat

-
+
Western blot analysis of BLCAP on recombinant protein tissue lysate using anti-BLCAP antibody at 1/5,000 dilution.
  • Western blot analysis of BLCAP on recombinant protein tissue lysate using anti-BLCAP antibody at 1/5,000 dilution.
  • ICC staining BLCAP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-BLCAP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-BLCAP antibody. Counter stained with hematoxylin.
Western blot analysis of BLCAP on recombinant protein tissue lysate using anti-BLCAP antibody at 1/5,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

BLCAP Mouse Monoclonal Antibody [4E1] (M1701-10)

Immunogen

Peptide

Host

Mouse

Positive Control

HepG2, rat cervix tissue, human breast tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

4E1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

BLCAP

SYNONYMS

Bc10 antibody; Bladder cancer 10 kDa protein antibody; Bladder cancer related protein (10kD) antibody; Bladder cancer-associated protein antibody; BLCAP antibody; BLCAP_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the BLCAP family.

TISSUE SPECIFICITY

Ubiquitous. Expressed in cervical tissues. Down-regulated in bladder invasive carcinoma, renal cell carcinoma and in primary cervical carcinoma.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

BLCAP is a highly conversed gene with two exons and an intron encoding a 10kDa protein, which was originally identified from invasive bladder carcinoma in 2002. BLCAP is subject to adenosine to inosine (A-to-I) RNA editing. A-to-I RNA editing is an important post-transcription modification of RNA sequence, generating a diversity of RNA products different from the original DNA sequence. The adenosine deaminase acting on double-stranded RNA (ADAR) family of enzymes catalyze the conversion of adenosine (A) to inosine (I) in the double stranded RNA, and inosine is finally recognized as guanosine (G) in the process of mRNA translation. May regulate cell proliferation and coordinate apoptosis and cell cycle progression via a novel mechanism independent of both p53/TP53 and NF-kappa-B.