His tag, AlpSdAbs® VHH(Biotin) Alpaca Monoclonal Antibody (HA710192)
Catalog# HA710192
Alpaca Monoclonal to His tag
Tested for
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Purification
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WB
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IF-Cell
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ELISA
Western blot analysis of His tag on different lysates with Alpaca anti-His tag, AlpSdAbs® VHH(Biotin) antibody (HA710192) at 1/5,000 dilution.
Lane 1: 293T transfected with His-tagged empty control cell lysate
Lane 2: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710192) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Streptavidin-HRP Secondary Antibody at 1/20,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with His-tagged empty control cell lysate
Lane 2: 293T transfected with His-tagged Histone H3.1 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA710192) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Streptavidin-HRP Secondary Antibody at 1/20,000 dilution was used for 1 hour at room temperature.