Biotin Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1101)
Catalog# HA1101
Goat Polyclonal to Goat anti-rabbit IgG
-
WB
-
ELISA
-
IHC-P
-
IF-Tissue
-
FC
-
IF-Cell
-
Rabbit
Lane 1: Rabbit IgG, 1:5,000
Lane 2: Rabbit IgG, 1:20,000
Lane 3: Mouse IgG, 1:5,000
Lane 4: Mouse IgG, 1:20,000
Lane 5: Human IgG, 1:5,000
Lane 6: Human IgG, 1:20,000
Lysates/proteins at 50 ng/Lane.
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Goat anti-rabbit IgG-biotin (HA1101) at different dilutions were used in 5% NFDM/TBST at 4℃ overnight. Streptavidin-HRP Secondary Antibody at 1/20,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Goat polyclonal secondary
Product Name
Biotin Conjugated Goat anti-rabbit IgG Goat Polyclonal Antibody (HA1101)
Immunogen
Rabbit igg (h+l).
Specificity
To minimize cross-reactivity, the biotin conjugated goat anti-rabbit igg (h+l) antibodies have been highly cross-adsorbed against human igg and mouse igg.
Host
Goat
Conjugation
Biotin
Clonality
Polyclonal
APPLICATION DILUTION
-
WB
-
1:5,000-1:20,000
-
ELISA
-
1:20,000-1:400,000
-
IHC-P
-
1:500-1:5,000
-
IF-Tissue
-
1:200-1:1,000
-
FC
-
1:200-1:1,000
-
IF-Cell
-
1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 1%BSA, 50%Glycerol.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
Goat anti-rabbit IgG
FUNCTION
Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.Biotin-SP is our trade name for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.
