Biotin Conjugated Human CXCL5 Recombinant Rabbit Monoclonal Antibody - Detector [PSH18-71] (HA725362B)
Catalog# HA725362B
Rabbit Monoclonal to CXCL5
Tested for
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ELISA(Det)
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ELISA
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to Biotin
Sandwich ELISA analysis of human CXCL5 matched pair antibodies
Capture: HA725361, Human CXCL5 Rabbit mAb [PSH18-70]
Detector: HA725362, Human CXCL5 Rabbit mAb [PSH18-71]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725361) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL5 protein (HA210887) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725362, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Capture: HA725361, Human CXCL5 Rabbit mAb [PSH18-70]
Detector: HA725362, Human CXCL5 Rabbit mAb [PSH18-71]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725361) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL5 protein (HA210887) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725362, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.