BHMT Mouse Monoclonal Antibody [9E1] (EM1701-64)
Catalog# EM1701-64
Mouse Monoclonal to BHMT
-
WB
-
IHC-P
-
IF-Cell
-
FC
-
Human
-
Mouse
-
Rat
Lane 1: Human liver tissue lysate
Lane 2: Human kidney tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 5 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-64) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
BHMT Mouse Monoclonal Antibody [9E1] (EM1701-64)
Immunogen
Recombinant protein with human bhmt aa 250-406 / 406.
Host
Mouse
Positive Control
Human liver tissue lysate, human kidney tissue lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue, SiHa, HepG2.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
9E1
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:20,000
-
IF-Cell
-
1:50
-
IHC-P
-
1:2,000
-
FC
-
1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
BHMT
SYNONYMS
Betaine homocysteine methyltransferase antibody;Betaine homocysteine S methyltransferase 1 antibody;Betaine homocysteine S methyltransferase antibody;Betaine--homocysteine S-methyltransferase 1 antibody;BHMT antibody;BHMT1 antibody;BHMT1_HUMAN antibody;EC 2.1.1.5 antibody;Epididymis secretory sperm binding protein Li 61p antibody;HEL S 61p antibody
TISSUE SPECIFICITY
Found exclusively in liver and kidney.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Betaine-homocysteine methyltransferase (BHMT) is a zinc-dependent cytosolic protein that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT is highly expressed in rat liver, and its expression is regulated by dietary methionine and choline. In humans, S-adenosylmethionine (SAM) down-regulates BHMT expression by inducing NFκB, which acts as a repressor for the BHMT gene. Lowered BHMT levels can lead to ER (endoplasmic reticulum) stress. Mutations in the gene encoding for BHMT may lead to hyperhomocysteinemia, a medical condition characterized by abnormally large amounts of homocysteine in the blood which may be a risk factor for cardiovascular and cerebrovascular diseases.
CITATIONS
-
Wang Y, Zhao Z, Yu H, et al
Stability and function of RCL1 are dependent on interaction with BMS1
-
IF
-
Zebrafish
-
Citation
-
