PRODUCT CODE: EM1701-64

BHMT Mouse Monoclonal Antibody [9E1] (EM1701-64)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of BHMT on human liver tissue and human kidney tissue lysate using anti-BHMT antibody at 1/1,000 dilution.
  • Western blot analysis of BHMT on human liver tissue and human kidney tissue lysate using anti-BHMT antibody at 1/1,000 dilution.
  • ICC staining BHMT (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining BHMT (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-BHMT antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-BHMT antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-BHMT antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with BHMT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG1 was used as the secondary antibody.
Western blot analysis of BHMT on human liver tissue and human kidney tissue lysate using anti-BHMT antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

BHMT Mouse Monoclonal Antibody [9E1] (EM1701-64)

Immunogen

Recombinant protein.

Host

Mouse

Positive Control

Human kidney tissue lysate, human liver tissue, HepG2, SiHa, rat kidney tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

9E1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50 IHC-P

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

BHMT

SYNONYMS

Betaine homocysteine methyltransferase antibody; Betaine homocysteine S methyltransferase 1 antibody; Betaine homocysteine S methyltransferase antibody; Betaine--homocysteine S-methyltransferase 1 antibody; BHMT antibody; BHMT1 antibody; BHMT1_HUMAN antibody; EC 2.1.1.5 antibody; Epididymis secretory sperm binding protein Li 61p antibody; HEL S 61p antibody

TISSUE SPECIFICITY

Found exclusively in liver and kidney.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Betaine-homocysteine methyltransferase (BHMT) is a zinc-dependent cytosolic protein that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. BHMT is highly expressed in rat liver, and its expression is regulated by dietary methionine and choline. In humans, S-adenosylmethionine (SAM) down-regulates BHMT expression by inducing NFκB, which acts as a repressor for the BHMT gene. Lowered BHMT levels can lead to ER (endoplasmic reticulum) stress. Mutations in the gene encoding for BHMT may lead to hyperhomocysteinemia, a medical condition characterized by abnormally large amounts of homocysteine in the blood which may be a risk factor for cardiovascular and cerebrovascular diseases.