Applications
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WB
REACTIVITY
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Human
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Mouse
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Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
beta III Tubulin Rabbit Polyclonal Antibody (0807-2)
Immunogen
Synthetic peptide within human beta-tubulin aa 401-450 / 450.
Host
Rabbit
Positive Control
Hela, PC12
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Immunogen affinity purified
MOLECULAR WEIGHT
50 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
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1:2,000
TARGET
UNIPROT #
PROTEIN NAME
beta III Tubulin
SYNONYMS
beta 3 tubulin antibody; beta-4 antibody; CDCBM antibody; CDCBM1 antibody; CFEOM3 antibody; CFEOM3A antibody; FEOM3 antibody; M(beta)3 antibody; M(beta)6 antibody; MC1R antibody; Neuron specific beta III Tubulin antibody; Neuron-specific class III beta-tubulin antibody; QccE-11995 antibody; QccE-15186 antibody; TBB3_HUMAN antibody; Tubb 3 antibody; TUBB3 antibody; TUBB4 antibody; Tubulin beta 3 antibody; Tubulin beta 3 chain antibody; Tubulin beta 4 antibody; Tubulin beta III antibody; Tubulin beta-3 chain antibody; Tubulin beta-4 chain antibody; Tubulin beta-III antibody
SEQUENCE SIMILARITIES
Belongs to the tubulin family.
TISSUE SPECIFICITY
Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues.
POST-TRANSLATIONAL MODIFICATION
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
SUBCELLULAR LOCATION
Microtubule
FUNCTION
Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP.
CITATIONS
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Jinyan Gu; Jiyong Zhou
Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid
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WB
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CoIP
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human
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Citation
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Lin, Cui et al.
Caspase-Dependent Apoptosis Induction via Viral Protein ORF4 of Porcine Circovirus 2 Binding to Mitochondrial Adenine Nucleotide Translocase 3. | Journal of Virology [2018]
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WB
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Porcine Circovirus
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Citation
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Zhou, J., Wang, Y., Qiu, Y....
Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein
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WB
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Pig
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Citation
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Liu, H., Lou, J., Liu, Y., ...
Intestinal epithelial cell autophagy deficiency suppresses inflammation-associated colon tumorigenesis
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WB
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Mouse
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Citation
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