PRODUCT CODE: ET1609-48

beta Ⅱ Tubulin Recombinant Rabbit Monoclonal Antibody [ST52-04] (ET1609-48)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of beta Ⅱ Tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: mouse brain tissue lysate<br />
Lane 2: mouse kidney tissue lysate
  • Western blot analysis of beta Ⅱ Tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: mouse brain tissue lysate<br />
Lane 2: mouse kidney tissue lysate
  • ICC staining of beta Ⅱ Tubulin in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1609-48, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-beta Ⅱ Tubulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta Ⅱ Tubulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta Ⅱ Tubulin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-48, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of beta Ⅱ Tubulin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: mouse brain tissue lysate
Lane 2: mouse kidney tissue lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

beta Ⅱ Tubulin Recombinant Rabbit Monoclonal Antibody [ST52-04] (ET1609-48)

Immunogen

Synthetic peptide within human beta ⅱ tubulin aa 407-445 / 445.

Host

Rabbit

Positive Control

Mouse brain tissue lysate, mouse kidney tissue lysate, N2A, mouse kidney tissue, mouse brain tissue, rat brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST52-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

beta Ⅱ Tubulin

SYNONYMS

bA506K6.1 antibody; Beta2 antibody; class II beta tubulin isotype antibody; class IIa beta-tubulin antibody; class IIb beta-tubulin antibody; Class IVb beta tubulin antibody; dJ40E16.7 antibody; DKFZp566F223 antibody; FLJ98847 antibody; M(beta)2 antibody; MGC8685 antibody; OTTHUMP00000015956 antibody; OTTHUMP00000015964 antibody; TBB4B_HUMAN antibody; TUBB 2 antibody; TUBB 2A antibody; TUBB 2C antibody; TUBB antibody; TUBB PARALOG antibody; TUBB2 antibody; TUBB2A antibody; TUBB2B antibody; TUBB2C antibody; Tubb4b antibody; Tubulin beta 2 antibody; Tubulin beta 2 chain antibody; Tubulin beta 2A antibody; Tubulin beta 2A chain antibody; Tubulin beta 2B antibody; Tubulin beta 2B chain antibody; Tubulin beta 2C antibody; Tubulin beta polypeptide antibody; Tubulin beta polypeptide 2 antibody; Tubulin beta polypeptide paralog antibody; Tubulin beta-2 chain antibody; Tubulin beta-2C chain antibody; Tubulin beta-4B chain antibody; tubulin, beta 2A class IIa antibody; tubulin, beta 2B class IIb antibody; tubulin, beta 4B class IVb antibody; Tubulin, beta, class IVB antibody; Tubulin, beta-4B antibody

SEQUENCE SIMILARITIES

Belongs to the tubulin family.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

SUBCELLULAR LOCATION

Cytoskeleton.

FUNCTION

Tubulin is a major cytoskeleton component that has five distinct forms, designated a, b, g, d and e tubulin. a and b tubulins form heterodimers which multimerize to form a microtubule filament. Multiple b Tubulin isoforms (b1, b2, b3, b4, b5, b6 and b8) have been characterized and are expressed in mammalian tissues. b1 and b4 are present throughout the cytosol, b2 is present in the nuclei and nucleoplasm, and b3 is a neuron-specific cytoskeletal protein. g Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both d Tubulin and e Tubulin are associated with the centrosome. d Tubulin is a homolog of the Chlamydomonas d Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes.