PRODUCT CODE: ER1802-13

Beta-Arrestin 1 Rabbit Polyclonal Antibody (ER1802-13)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of beta Arrestin 1 on human placenta tissue lysate using anti-beta Arrestin 1 antibody at 1/500 dilution.
  • Western blot analysis of beta Arrestin 1 on human placenta tissue lysate using anti-beta Arrestin 1 antibody at 1/500 dilution.
  • ICC staining beta Arrestin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining beta Arrestin 1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining beta Arrestin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta Arrestin 1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Jurkat cells with beta Arrestin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of beta Arrestin 1 on human placenta tissue lysate using anti-beta Arrestin 1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Beta-Arrestin 1 Rabbit Polyclonal Antibody (ER1802-13)

Immunogen

Synthetic peptide within n-terminal human beta-arrestin 1.

Host

Rabbit

Positive Control

Human placenta tissue lysate, A549, HUVEC, LOVO, rat brain tissue, human colon cancer tissue, human breast tissue, mouse colon tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

50 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Beta-Arrestin 1

SYNONYMS

ARB1 antibody; ARR1 antibody; ARRB1 antibody; ARRB1_HUMAN antibody; Arrestin 2 antibody; Arrestin beta 1 antibody; Arrestin beta-1 antibody; Beta-arrestin-1 antibody

SEQUENCE SIMILARITIES

Belongs to the arrestin family.

POST-TRANSLATIONAL MODIFICATION

Constitutively phosphorylated at Ser-412 in the cytoplasm. At the plasma membrane, is rapidly dephosphorylated, a process that is required for clathrin binding and ADRB2 endocytosis but not for ADRB2 binding and desensitization. Once internalized, is rephosphorylated.; The ubiquitination status appears to regulate the formation and trafficking of beta-arrestin-GPCR complexes and signaling. Ubiquitination appears to occur GPCR-specific. Ubiquitinated by MDM2; the ubiquitination is required for rapid internalization of ADRB2. Deubiquitinated by USP33; the deubiquitination leads to a dissociation of the beta-arrestin-GPCR complex. Stimulation of a class A GPCR, such as ADRB2, induces transient ubiquitination and subsequently promotes association with USP33.

SUBCELLULAR LOCATION

Cytoplasm. Membrane. Nucleus.

FUNCTION

The members of the G protein coupled receptor family are distinguished by their slow transmitting response to ligand binding. These seven transmembrane proteins include the adrenergic, serotonin and dopamine receptors. The effect of the signaling molecule can be excitatory or inhibitory depending on the type of receptor to which it binds. Members of the β-Arrestin family regulate receptor binding to G proteins. β-Arrestins have been found to be located at postsynaptic sites, where they are thought to act in concert with βARK (βARK1, also designated GRK 2, or βARK2, also designated GRK 3) to regulate G protein-coupled neurotransmitter receptors. Expression of β-Arrestin-1 and b-Arrestin-2 is seen predominantly in spleen and neuronal tissues. It has been shown that β-Arrestin-1 expression is modulated by intracellular cAMP, which may be a novel mechanism for the regulation of receptor-mediated responses.