PRODUCT CODE: ET1702-67

beta Actin (HRP conjugated) Recombinant Rabbit Monoclonal Antibody [JF53-10] (ET1702-67)

  • Zebrafish
  • Recombinant

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Monkey

  • Hamster

  • Zebrafish

Western blot analysis of beta Actin (HRP conjugated) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-67, 1/500) was used in 5% BSA at room temperature for 2 hours.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: PC-12 cell lysate<br />
Lane 3: NIH/3T3 cell lysate<br />
Lane 4: zebrafish tissue lysate
  • Western blot analysis of beta Actin (HRP conjugated) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-67, 1/500) was used in 5% BSA at room temperature for 2 hours.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: PC-12 cell lysate<br />
Lane 3: NIH/3T3 cell lysate<br />
Lane 4: zebrafish tissue lysate
  • Western blot analysis of beta Actin (HRP conjugated) on hybrid fish (crucian-carp) brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-67, 1/500) was used in 5% BSA at room temperature for 2 hours.
Western blot analysis of beta Actin (HRP conjugated) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-67, 1/500) was used in 5% BSA at room temperature for 2 hours.
Positive control:
Lane 1: Hela cell lysate
Lane 2: PC-12 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: zebrafish tissue lysate

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Monkey

  • Hamster

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

beta Actin (HRP conjugated) Recombinant Rabbit Monoclonal Antibody [JF53-10] (ET1702-67)

Immunogen

Recombinant full length protein of human beta actin.

Host

Rabbit

Positive Control

Hela cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, zebrafish tissue lysate, hybrid fish (crucian-carp) brain tissue lysates.

Conjugation

Hrp

Clonality

Monoclonal

Clone Number

JF53-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

beta Actin (HRP conjugated)

SYNONYMS

A26C1A antibody; A26C1B antibody; ACTB antibody; ACTB_HUMAN antibody; Actin beta antibody; Actin cytoplasmic 1 antibody; Actin, cytoplasmic 1, N-terminally processed antibody; Actx antibody; b actin antibody; Beta cytoskeletal actin antibody; Beta-actin antibody; BRWS1 antibody; E430023M04Rik antibody; MGC128179 antibody; PS1TP5 binding protein 1 antibody; PS1TP5BP1 antibody

SEQUENCE SIMILARITIES

Belongs to the actin family.

POST-TRANSLATIONAL MODIFICATION

ISGylated.; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

CITATIONS

  • Dong, Aili et al.

    Protective effects of hydrogen gas against sepsis-induced acute lung injury via regulation of mitochondrial function and dynamics. | International Immunopharmacology [2018]

  • Wang, Qianting et al.

    Fusobacterium nucleatum stimulates monocyte adhesion to and transmigration through endothelial cells. | Archives of Oral Biology [2019]