PRODUCT CODE: M1210-5

HRP Conjugated beta Actin Mouse Monoclonal Antibody [A2-F6] (M1210-5)

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of beta Actin (HRP conjugated) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1210-5, 1/1,000) was used in 5% BSA at room temperature for 2 hours.<br />
Positive control: <br />
Lane 1: PC12 cell lysate<br />
Lane 2: Hela cell lysate<br />
Lane 3: NIH/3T3 cell lysate
  • Western blot analysis of beta Actin (HRP conjugated) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1210-5, 1/1,000) was used in 5% BSA at room temperature for 2 hours.<br />
Positive control: <br />
Lane 1: PC12 cell lysate<br />
Lane 2: Hela cell lysate<br />
Lane 3: NIH/3T3 cell lysate
Western blot analysis of beta Actin (HRP conjugated) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1210-5, 1/1,000) was used in 5% BSA at room temperature for 2 hours.
Positive control:
Lane 1: PC12 cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH/3T3 cell lysate

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

HRP Conjugated beta Actin Mouse Monoclonal Antibody [A2-F6] (M1210-5)

Immunogen

Synthetic peptide within n-terminal human beta-actin .

Host

Mouse

Positive Control

PC12 cell lysate, Hela cell lysate, NIH/3T3 cell lysate.

Conjugation

Hrp

Clonality

Monoclonal

Clone Number

A2-F6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

TARGET

UNIPROT #

PROTEIN NAME

beta Actin (HRP conjugated)

SYNONYMS

A26C1A antibody; A26C1B antibody; ACTB antibody; ACTB_HUMAN antibody; Actin beta antibody; Actin cytoplasmic 1 antibody; Actin, cytoplasmic 1, N-terminally processed antibody; Actx antibody; b actin antibody; Beta cytoskeletal actin antibody; Beta-actin antibody; BRWS1 antibody; E430023M04Rik antibody; MGC128179 antibody; PS1TP5 binding protein 1 antibody; PS1TP5BP1 antibody

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.

CITATIONS

  • Hongqiang Cheng;

    Shp2 in myocytes is essential for cardiovascular and neointima development.