Beta actin Mouse Monoclonal Antibody [A2-F6] (M1210-2)


  • WB

  • ICC

  • IHC-P

  • FC


  • Human

  • Mouse

  • Rat

Immunofluorescent staining of NIH/3T3 cells using anti-beta-actin mouse mAb.
  • Immunofluorescent staining of NIH/3T3 cells using anti-beta-actin mouse mAb.
  • Western blot analysis of Beta-actin on different cell lysates using anti- Beta-actin antibody at 1/10000 dilution.<br />
Positive control:<br />
Lane 1: NIH/3T3<br />
Lane 2: PC12<br />
Lane 3: MCF-7<br />
Lane 4: HepG2<br />
Lane 5: Hela<br />
Lane 6: Mouse lung
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
Immunofluorescent staining of NIH/3T3 cells using anti-beta-actin mouse mAb.


  • WB

  • ICC

  • IHC-P

  • FC


  • Human

  • Mouse

  • Rat


Product Type

Mouse monoclonal primary

Product Name

Beta actin Mouse Monoclonal Antibody [A2-F6] (M1210-2)


Synthetic peptide within n-terminal of human beta antin.



Positive Control

NIH/3T3, PC12, MCF-7, HepG2, Hela, human colon





Clone Number





Storage Condition

Store at -20 C. Stable for 12 months from date of receipt.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.


2 ug/ul


Peptide affinity purified.






  • WB

  • 1:10,000

  • ICC

  • 1:100-1:200

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:100-1:200




Beta actin


A26C1A antibody; A26C1B antibody; ACTB antibody; ACTB_HUMAN antibody; Actin beta antibody; Actin cytoplasmic 1 antibody; Actin, cytoplasmic 1, N-terminally processed antibody; Actx antibody; b actin antibody; Beta cytoskeletal actin antibody; Beta-actin antibody; BRWS1 antibody; E430023M04Rik antibody; MGC128179 antibody; PS1TP5 binding protein 1 antibody; PS1TP5BP1 antibody


Belongs to the actin family.


ISGylated.; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.




Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.


  • Wang, Fei et al.

    Telocytes in liver regeneration: possible roles. | Journal of Cellular and Molecular Medicine [2014]

  • Yu, Jiekai et al.

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  • Liu, Jiang et al.

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  • Li, Ping et al.

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  • Zhao, S-J et al.

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  • Lu, Meng et al.

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  • Yang, Fan et al.

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  • Zhao, Shu-Jie et al.

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  • Liang, Ruichao et al.

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  • Cai, Jiaoyang et al.

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  • Xu, Yanli et al.

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  • Guo, Chuansheng et al.

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  • Hou, Mingyu et al.

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  • Shen, Yifei et al.

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  • Lu, Huan et al.

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  • Li, Yi-Ming et al.

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  • Li, Jialun et al.

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  • Zhang, Fanghui et al.

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  • Yang, Dahai et al.

    The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection. | PloS Pathogens [2019]

  • Liu, Xia et al.

    Silencing RRM2 inhibits multiple myeloma by��targeting the Wnt/β-catenin signaling pathway. | Molecular Medicine Reports [2019]

  • Heybrock, Saskia et al.

    Lysosomal integral membrane protein-2 (LIMP-2/SCARB2) is involved in lysosomal cholesterol export. | Nature Communications [2019]

  • Yu, Weiwei et al.

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  • Li, Yi-Ming et al.

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  • Lu, Yan et al.

    Palmitoylation of NOD1 and NOD2 is required for bacterial sensing. | Science (New York, N.Y.) [2019]

  • Qiu, Qiang et al.

    Targeting glutaminase1 and synergizing with clinical drugs achieved more promising antitumor activity on multiple myeloma. | Oncotarget [2019]

  • Shangguan, Wenbing et al.

    FoxD2-AS1 is a prognostic factor in glioma and promotes temozolomide resistance in a O6-methylguanine-DNA methyltransferase-dependent manner. | The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology [2019]

  • Wang, Han et al.

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  • Zhang, Meng-Yi et al.

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  • Zhang, Bo et al.

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  • Lv, Dandan et al.

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  • Li, Yang et al.

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  • Zhou, Jianwei et al.

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