PRODUCT CODE: M1210-2

Beta actin Mouse Monoclonal Antibody [A2-F6] (M1210-2)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Immunofluorescent staining of NIH/3T3 cells using anti-beta-actin mouse mAb.
  • Immunofluorescent staining of NIH/3T3 cells using anti-beta-actin mouse mAb.
  • Western blot analysis of Beta-actin on different cell lysates using anti- Beta-actin antibody at 1/10000 dilution.<br />
Positive control:<br />
Lane 1: NIH/3T3<br />
Lane 2: PC12<br />
Lane 3: MCF-7<br />
Lane 4: HepG2<br />
Lane 5: Hela<br />
Lane 6: Mouse lung
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
Immunofluorescent staining of NIH/3T3 cells using anti-beta-actin mouse mAb.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Beta actin Mouse Monoclonal Antibody [A2-F6] (M1210-2)

Immunogen

Synthetic peptide within n-terminal of human beta antin.

Host

Mouse

Positive Control

NIH/3T3, PC12, MCF-7, HepG2, Hela, human colon

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A2-F6

PROPERTIES

Form

Liquid

Storage Condition

Store at -20 C. Stable for 12 months from date of receipt.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

43kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:10,000

  • ICC

  • 1:100-1:200

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Beta actin

SYNONYMS

A26C1A antibody; A26C1B antibody; ACTB antibody; ACTB_HUMAN antibody; Actin beta antibody; Actin cytoplasmic 1 antibody; Actin, cytoplasmic 1, N-terminally processed antibody; Actx antibody; b actin antibody; Beta cytoskeletal actin antibody; Beta-actin antibody; BRWS1 antibody; E430023M04Rik antibody; MGC128179 antibody; PS1TP5 binding protein 1 antibody; PS1TP5BP1 antibody

SEQUENCE SIMILARITIES

Belongs to the actin family.

POST-TRANSLATIONAL MODIFICATION

ISGylated.; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Beta actin also know as Cytoplasmic Actin is a 43 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

CITATIONS

  • Wang, Fei et al.

    Telocytes in liver regeneration: possible roles. | Journal of Cellular and Molecular Medicine [2014]

  • Yu, Jiekai et al.

    High-throughput proteomics integrated with gene microarray for discovery of colorectal cancer potential biomarkers. | Oncotarget [2016]

  • Liu, Jiang et al.

    A critical role of DDRGK1 in endoplasmic reticulumhomoeostasis via regulation of IRE1a stability. | Nature Communications [2017]

  • Li, Ping et al.

    Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions. | Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms [2017]

  • Zhao, S-J et al.

    SPARCL1 suppresses osteosarcoma metastasis and recruits macrophages by activation of canonical WNT/β-catenin signaling through stabilization of the WNT-receptor complex. | Oncogene [2018]

  • Lu, Meng et al.

    Basolateral CD147 induces hepatocyte polarity loss by E-cadherin ubiquitination and degradation in hepatocellular carcinoma progress. | Hepatology (Baltimore, Md.) [2018]

  • Yang, Fan et al.

    HPRT1 activity loss is associated with resistance to thiopurine in ALL. | Oncotarget [2018]

  • Zhao, Shu-Jie et al.

    SLIT2/ROBO1 axis contributes to the Warburg effect in osteosarcoma through activation of SRC/ERK/c-MYC/PFKFB2 pathway. | Cell Death & Disease [2018]

  • Yu, Jiekai et al.

    Identification of Kininogen 1 as a Serum Protein Marker of Colorectal Adenoma in Patients with a Family History of Colorectal Cancer. | Journal of Cancer [2018]

  • Cao, Huifang et al.

    Edwardsiella piscicida Type III Secretion System Effector EseK Inhibits Mitogen-Activated Protein Kinase Phosphorylation and Promotes Bacterial Colonization in Zebrafish Larvae. | Infection and Immunity [2018]

  • Liang, Ruichao et al.

    Macrophage Polarization in Response to Varying Pore Sizes of 3D Polyurethane Scaffolds. | Journal of Biomedical Nanotechnology [2018]

  • Cai, Jiaoyang et al.

    A novel fusion gene PLEKHA6-NTRK3 in langerhans cell histiocytosis. | International Journal of Cancer [2019]

  • Xu, Yanli et al.

    Transcription coactivator Cited1 acts as an inducer of trophoblast-like state from mouse embryonic stem cells through the activation of BMP signaling. | Cell Death & Disease [2018]

  • Guo, Chuansheng et al.

    Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]

  • Hou, Mingyu et al.

    Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner. | Infection and Immunity [2019]

  • Shen, Yifei et al.

    S1P/S1PR3 axis promotes aerobic glycolysis by YAP/c-MYC/PGAM1 axis in osteosarcoma. | EbioMedicine [2019]

  • Lu, Huan et al.

    Targeting cancer stem cell signature gene SMOC-2 Overcomes chemoresistance and inhibits cell proliferation of endometrial carcinoma. | EbioMedicine [2019]

  • Li, Yi-Ming et al.

    Alterations of the Immunologic Co-Stimulator B7 and TNFR Families Correlate with Hepatocellular Carcinoma Prognosis and Metastasis by Inactivating STAT3. | International Journal of Molecular Sciences [2019]

  • Li, Jialun et al.

    Activated MEK/ERK Pathway Drives Widespread and Coordinated Overexpression of UHRF1 and DNMT1 in Cancer cells. | Scientific Reports [2019]

  • Zhang, Fanghui et al.

    Specific Decrease in B-Cell-Derived Extracellular Vesicles Enhances Post-Chemotherapeutic CD8+ T Cell Responses. | Immunity [2019]

  • Yang, Dahai et al.

    The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection. | PloS Pathogens [2019]

  • Liu, Xia et al.

    Silencing RRM2 inhibits multiple myeloma by��targeting the Wnt/β-catenin signaling pathway. | Molecular Medicine Reports [2019]

  • Heybrock, Saskia et al.

    Lysosomal integral membrane protein-2 (LIMP-2/SCARB2) is involved in lysosomal cholesterol export. | Nature Communications [2019]

  • Yu, Weiwei et al.

    One-Carbon Metabolism Supports S-Adenosylmethionine and Histone Methylation to Drive Inflammatory Macrophages. | Molecular Cell [2019]

  • Li, Yi-Ming et al.

    Programmed Death Ligand 1 Indicates Pre-Existing Adaptive Immune Response by Tumor-Infiltrating CD8+ T Cells in Non-Small Cell Lung Cancer. | International Journal of Molecular Sciences [2019]

  • Lu, Yan et al.

    Palmitoylation of NOD1 and NOD2 is required for bacterial sensing. | Science (New York, N.Y.) [2019]

  • Qiu, Qiang et al.

    Targeting glutaminase1 and synergizing with clinical drugs achieved more promising antitumor activity on multiple myeloma. | Oncotarget [2019]

  • Shangguan, Wenbing et al.

    FoxD2-AS1 is a prognostic factor in glioma and promotes temozolomide resistance in a O6-methylguanine-DNA methyltransferase-dependent manner. | The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology [2019]

  • Wang, Han et al.

    The role of sphingosine-1-phosphate signaling pathway in cementocyte mechanotransduction. | Biochemical and Biophysical Research Communications [2020]

  • Zhang, Meng-Yi et al.

    Bromodomains and Extra-Terminal (BET) Inhibitor JQ1 Suppresses Proliferation of Acute Lymphocytic Leukemia by Inhibiting c-Myc-Mediated Glycolysis. | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research [2020]

  • Zhang, Bo et al.

    Cooperative transport mechanism of human monocarboxylate transporter 2. | Nature Communications [2020]

  • Lv, Dandan et al.

    A novel cell-based assay for dynamically detecting neutrophil extracellular traps-induced lung epithelial injuries. | Experimental Cell Research [2020]

  • Li, Yang et al.

    The function of a heterozygous p53 mutation in a Li-Fraumeni syndrome patient. | PloS One [2020]

  • Zhou, Jianwei et al.

    Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins. | Frontiers in Microbiology [2020]