PRODUCT CODE: EM21002

Beta Actin Mouse Monoclonal Antibody [A2-F6] (EM21002)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution.<br />
Positive control:<br />
Lane 1: NIH/3T3 cell lysate<br />
Lane 2: PC12 cell lysate<br />
Lane 3: MCF-7 cell lysate<br />
Lane 4: HepG2 cell lysate<br />
Lane 5: Hela cell lysate<br />
Lane 6: Mouse lung tissue lysate
  • Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution.<br />
Positive control:<br />
Lane 1: NIH/3T3 cell lysate<br />
Lane 2: PC12 cell lysate<br />
Lane 3: MCF-7 cell lysate<br />
Lane 4: HepG2 cell lysate<br />
Lane 5: Hela cell lysate<br />
Lane 6: Mouse lung tissue lysate
  • ICC staining of Beta-actin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of Beta-actin in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of Beta-actin in NIH-3T3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Beta-actin antibody. Counter stained with hematoxylin.
Western blot analysis of Beta-actin on different lysates using anti-Beta-actin antibody at 1/10,000 dilution.
Positive control:
Lane 1: NIH/3T3 cell lysate
Lane 2: PC12 cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: Hela cell lysate
Lane 6: Mouse lung tissue lysate

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Beta Actin Mouse Monoclonal Antibody [A2-F6] (EM21002)

Immunogen

Synthetic peptide (klh-coupled) within human beta-actin n terminal.

Host

Mouse

Positive Control

NIH/3T3, PC12, MCF-7, HepG2, Hela, mouse lung A549, human colon carcinoma tissue, mouse prostate tissue , mouse kidney tissue

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A2-F6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:10,000

  • ICC

  • 1:100-1:200

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Beta Actin

SYNONYMS

A26C1A antibody; A26C1B antibody; ACTB antibody; ACTB_HUMAN antibody; Actin beta antibody; Actin cytoplasmic 1 antibody; Actin, cytoplasmic 1, N-terminally processed antibody; Actx antibody; b actin antibody; Beta cytoskeletal actin antibody; Beta-actin antibody; BRWS1 antibody; E430023M04Rik antibody; MGC128179 antibody; PS1TP5 binding protein 1 antibody; PS1TP5BP1 antibody

SEQUENCE SIMILARITIES

Belongs to the actin family.

POST-TRANSLATIONAL MODIFICATION

ISGylated.; Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.; Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.; Methylated at His-73 by SETD3. Methylation at His-73 is required for smooth muscle contraction of the laboring uterus during delivery (By similarity).; [Actin, cytoplasmic 1, N-terminally processed]: N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.; (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Beta actin also know as Cytoplasmic Actin is a 42 kDa, highly conserved protein, ubiquitously expressed in all eukaryotic cells. They comprise, along with microtubules, a major component of the cytoskeleton. β-Actin is a relatively stable cytoskeletal protein normally at a constant level in cells, regardless of experimental treatment or technical procedure. For this reason, measurement of β -Actin is generally used as an internal control for experimental error.

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