beta 2 Adrenergic Receptor Recombinant Rabbit Monoclonal Antibody [JM102-06] (ET1703-04)
Catalog# ET1703-04
Rabbit Monoclonal to beta 2 Adrenergic Receptor
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WB
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IHC-P
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IP
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IF-Cell
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FC
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IHC-Fr
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Human
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Mouse
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Rat
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Zebrafish
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (ET1703-04, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
beta 2 Adrenergic Receptor Recombinant Rabbit Monoclonal Antibody [JM102-06] (ET1703-04)
Immunogen
Synthetic peptide within human adrb2 aa 369-400 / 413.
Host
Rabbit
Positive Control
A431 cell lysate, human liver tissue lysate, mouse heart tissue lysate, rat heart tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, zebrafish tissue lysates, A431, human liver tissue, human stomach tissue, mouse liver tissue, mouse stomach tissue, rat liver tissue, rat stomach tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM102-06
RRID
APPLICATION DILUTION
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WB
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1:5,000-1:20,000
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IHC-P
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1:200-1:5,000
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IP
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Use at an assay dependent concentration.
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IF-Cell
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1:100
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FC
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1:1,000
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IHC-Fr
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 46 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
beta 2 Adrenergic Receptor
SYNONYMS
ADRB2 antibody;ADRB2_HUMAN antibody;ADRB2R antibody;ADRBR antibody;Adrenergic beta 2 receptor surface antibody;Adrenoceptor beta 2 surface antibody;B2AR antibody;BAR antibody;beta 2 adrenoceptor antibody;Beta 2 adrenoreceptor antibody;Beta-2 adrenergic receptor antibody;Beta-2 adrenoceptor antibody;Beta-2 adrenoreceptor antibody;BETA2AR antibody;Catecholamine receptor antibody;OTTHUMP00000160386 antibody
SEQUENCE SIMILARITIES
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily.
POST-TRANSLATIONAL MODIFICATION
Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.; Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK.; Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.; Polyubiquitinated. Agonist-induced ubiquitination leads to sort internalized receptors to the lysosomes for degradation. Deubiquitination by USP20 and USP33, leads to ADRB2 recycling and resensitization after prolonged agonist stimulation. USP20 and USP33 are constitutively associated and are dissociated immediately after agonist stimulation. Ubiquitination by the VHL-E3 ligase complex is oxygen-dependent.; Hydroxylation by EGLN3 occurs only under normoxia and increases the interaction with VHL and the subsequent ubiquitination and degradation of ADRB2.
SUBCELLULAR LOCATION
Cell membrane, Early endosome.
FUNCTION
The beta-2 adrenergic receptor (β2 adrenoreceptor), also known as ADRB2, is a cell membrane-spanning beta-adrenergic receptor that binds epinephrine (adrenaline), a hormone and neurotransmitter whose signaling, via adenylate cyclase stimulation through trimeric Gs proteins, increased cAMP, and downstream L-type calcium channel interaction, mediates physiologic responses such as smooth muscle relaxation and bronchodilation. The ADRB2 gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes.
CITATIONS
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Koda, S., Zhang, B., Zhou, Q. Y., Xu, N., Li, J., Liu, J. X., Liu, M., Lv, Z. Y., Wang, J. L., Shi, Y., Gao, S., Yu, Q., Li, X. Y., Xu, Y. H., Chen, J. X., Tekengne, B., Adzika, G. K., Tang, R. X., Sun, H., Zheng, K. Y., … Yan, C.
β2-Adrenergic Receptor Enhances the Alternatively Activated Macrophages and Promotes Biliary Injuries Caused by Helminth Infection
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IHC
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WB
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Mouse
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Citation
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