PRODUCT CODE: ER130915

BDNF Rabbit Polyclonal Antibody (ER130915)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of BDNF on different cell lysates using anti-BDNF antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: A172<br />
Lane 2: SHG-44<br />
Lane 3: Mouse heart  Lane 4: Mouse brain
  • Western blot analysis of BDNF on different cell lysates using anti-BDNF antibody at 1/500 dilution.<br />
Positive control:<br />
Lane 1: A172<br />
Lane 2: SHG-44<br />
Lane 3: Mouse heart  Lane 4: Mouse brain
  • ICC staining of BNDF in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BDNF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-BDNF antibody. Counter stained with hematoxylin.
Western blot analysis of BDNF on different cell lysates using anti-BDNF antibody at 1/500 dilution.
Positive control:
Lane 1: A172
Lane 2: SHG-44
Lane 3: Mouse heart Lane 4: Mouse brain

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

BDNF Rabbit Polyclonal Antibody (ER130915)

Immunogen

Synthetic peptide within n-terminal human bdnf.

Host

Rabbit

Positive Control

A172, SHG-44, mouse heart, mouse brain, human lung tissue, mouse lung tissue, mouse testis tissue, mouse heart tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

28kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

BDNF

SYNONYMS

Abrineurin antibody; ANON2 antibody; BDNF antibody; BDNF_HUMAN antibody; Brain Derived Neurotrophic Factor antibody; Brain-derived neurotrophic factor antibody; BULN2 antibody; MGC34632 antibody; Neurotrophin antibody

SEQUENCE SIMILARITIES

Belongs to the NGF-beta family.

TISSUE SPECIFICITY

Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

POST-TRANSLATIONAL MODIFICATION

[BDNF precursor form]: N-glycosylated and glycosulfated, contrary to mature BDNF.; Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF. Can be converted into mature BDNF by plasmin (PLG).

SUBCELLULAR LOCATION

Secreted

FUNCTION

Brain-derived neurotrophic factor, also known as BDNF, is a member of the "neurotrophin" family of growth factors, which are related to the canonical "Nerve Growth Factor", NGF. BDNF acts on certain neurons of the central nervous system and the peripheral nervous system, helping to support the survival of existing neurons, and encourage the growth and differentiation of new neurons and synapses. BDNF is actually found in a range of tissue and cell types, not just in the brain. It is also expressed in the retina, the central nervous system, motor neurons, the kidneys, and the prostate. Various studies have shown possible links between BDNF and conditions such as depression, bipolar disorder,schizophrenia, obsessive-compulsive disorder, Alzheimer's disease, Huntington's disease, Rett syndrome, and dementia, as well as anorexia nervosa and bulimia nervosa.