Bcl-XL Recombinant Rabbit Monoclonal Antibody [SZ3-03] (ET1603-28)
Catalog# ET1603-28
Rabbit Monoclonal to Bcl-XL
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: K-562 cell lysate (10 µg/Lane)
Lane 2: Jurkat cell lysate (10 µg/Lane)
Lane 3: B16-F1 cell lysate (10 µg/Lane)
Lane 4: RAW264.7 cell lysate (10 µg/Lane)
Lane 5: C6 cell lysate (10 µg/Lane)
Lane 6: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 26 kDa
Observed band size: 30 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Bcl-XL Recombinant Rabbit Monoclonal Antibody [SZ3-03] (ET1603-28)
Immunogen
Synthetic peptide within human bcl-xl aa 37-86 / 233.
Host
Rabbit
Positive Control
K-562 cell lysate, Jurkat cell lysate, B16-F1 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Rat brain tissue lysate, K-562, human kidney tissue, mouse kidney tissue, rat kidney tissue, human colon carcinoma tissue, human breast carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SZ3-03
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 26 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Bcl-XL
SYNONYMS
Apoptosis regulator Bcl X antibody;Apoptosis regulator Bcl-X antibody;Apoptosis regulator BclX antibody;B cell lymphoma 2 like antibody;B2CL1_HUMAN antibody;Bcl 2 like 1 protein antibody;Bcl X antibody;Bcl xL antibody;BCL XL/S antibody;Bcl xS antibody;Bcl-2-like protein 1 antibody;Bcl2 Like 1 antibody;Bcl2 related gene antibody;Bcl2-L-1 antibody;BCL2L antibody;Bcl2l1 antibody;BCLX antibody;BclXL antibody;BclXs antibody;DKFZp781P2092 antibody;PPP1R52 antibody;Protein phosphatase 1 regulatory subunit 52 antibody
SEQUENCE SIMILARITIES
Belongs to the Bcl-2 family.
TISSUE SPECIFICITY
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
POST-TRANSLATIONAL MODIFICATION
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.; Phosphorylated on Ser-62 by CDK1. This phosphorylation is partial in normal mitotic cells, but complete in G2-arrested cells upon DNA-damage, thus promoting subsequent apoptosis probably by triggering caspases-mediated proteolysis. Phosphorylated by PLK3, leading to regulate the G2 checkpoint and progression to cytokinesis during mitosis. Phosphorylation at Ser-49 appears during the S phase and G2, disappears rapidly in early mitosis during prometaphase, metaphase and early anaphase, and re-appears during telophase and cytokinesis.; Ubiquitinated by RNF183 during prolonged ER stress, leading to degradation by the proteosome.
SUBCELLULAR LOCATION
Mitochondrion inner membrane, Mitochondrion outer membrane, Mitochondrion matrix, Cytoplasmic vesicle, Cytoplasm, Nucleus membrane.
FUNCTION
It is a well-established concept in the field of apoptosis that relative amounts of pro- and anti-survival Bcl-2 family of proteins determine whether the cell will undergo cell death; if more Bcl-xL is present, then pores are non-permeable to pro-apoptotic molecules and the cell survives. However, if Bax and Bak become activated, and Bcl-xL is sequestered away by gatekeeper BH3-only factors (e.g. Bim) causing a pore to form, cytochrome c is released leading to initiation of caspase cascade and apoptotic events. While the exact signaling pathway of Bcl-xL is still not known, it is believed that Bcl-xL differs highly from Bcl-2 in their mechanism of inducing apoptosis. Bcl-xL is about ten times more functional than Bcl-2 when induced by the chemotherapy drug, Doxorubicin and can specifically bind to cytochrome C residues, preventing apoptosis. It can also prevent the formation of Apaf-1 and Caspase 9 complex by acting directly upon Apaf-1 rather than Caspase 9, as shown in nematode homologs.
CITATIONS
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Mengyuan Li , Shuwen Zheng , Qinyuan Gong , Haifeng Zhuang , Zhaoxing Wu , Ping Wang , Xuzhao Zhang , Rongzhen Xu
An oral triple pill-based cocktail effectively controls acute myeloid leukemia with high translation
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WB
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Human
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Citation
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A network pharmacology approach to investigate dehydrocostus lactone inhibits the proliferation and epithelial-mesenchymal transition of human gastric cancer cells via regulating the PI3K/Akt and extracellular signal-regulated kinases/mitogen-activated protein kinase signalling pathways
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WB
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Human
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Citation
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Chloroquine Intervenes Nephrotoxicity of Nilotinib through Deubiquitinase USP13‐Mediated Stabilization of Bcl‐XL
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IHC
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IF
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IP
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WB
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Mouse
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Citation
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Meiqi Wan,et al
Rhein induces apoptosis of AGS and MGC803 cells by regulating the Ras/PI3K/AKT and p38/MAPK signaling pathway
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WB
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Mouse
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Citation
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Connexin 43 hyper-phosphorylation at serine 282 triggers apoptosis in rat cardiomyocytes via activation of mitochondrial apoptotic pathway
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WB
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Rat
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Citation
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Wang, A., Li, L., Li, M., Wang, S., & Wang, C.
Case Report: 7-Ethyl-10-Hydroxycamptothecin, a DNA Topoisomerase I Inhibitor, Performs BRD4 Inhibitory Activity and Inhibits Human Leukemic Cell Growth. Frontiers in pharmacology, 12, 664176.
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WB
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Citation
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