PRODUCT CODE: ER1901-74

BCL-6 Rabbit Polyclonal Antibody (ER1901-74)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

Western blot analysis of BCL-6 on Raji cell  lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of BCL-6 on Raji cell  lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining of BCL-6 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-74, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  • Flow cytometric analysis of BCL-6 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-74, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; blcak).
Western blot analysis of BCL-6 on Raji cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

BCL-6 Rabbit Polyclonal Antibody (ER1901-74)

Immunogen

Recombinant protein within human bcl-6 aa 287-464 / 706.

Host

Rabbit

Positive Control

Raji cell lysates, HepG2, Jurkat.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

79 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

BCL-6

SYNONYMS

B cell CLL/lymphoma 6 antibody; B cell lymphoma 6 protein antibody; B-cell lymphoma 5 protein antibody; B-cell lymphoma 6 protein antibody; BCL 5 antibody; Bcl 6 antibody; BCL-5 antibody; BCL-6 antibody; BCL5 antibody; BCL6 antibody; BCL6_HUMAN antibody; BCL6A antibody; cys his2 zinc finger transcription factor antibody; cys-his2 zinc finger transcription factor antibody; LAZ 3 antibody; LAZ 3 protein antibody; LAZ3 antibody; Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) antibody; Lymphoma associated zinc finger gene on chromosome 3 antibody; Protein LAZ-3 antibody; ZBTB 27 antibody; ZBTB27 antibody; Zinc finger and BTB domain containing protein 27 antibody; Zinc finger and BTB domain-containing protein 27 (ZBTB27) antibody; Zinc finger and BTB domain-containing protein 27 antibody; Zinc Finger Protein 51 (ZNF51) antibody; Zinc finger protein 51 antibody; zinc finger transcription factor BCL6S antibody; ZNF 51 antibody; ZNF51 antibody

TISSUE SPECIFICITY

Expressed in germinal center T- and B-cells and in primary immature dendritic cells.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by MAPK1 in response to antigen receptor activation at Ser-333 and Ser-343. Phosphorylated by ATM in response to genotoxic stress. Phosphorylation induces its degradation by ubiquitin/proteasome pathway.; Polyubiquitinated. Polyubiquitinated by SCF(FBXO11), leading to its degradation by the proteasome. Ubiquitinated by the SCF(FBXL17) complex, leading to its degradation by the proteaseome: ubiquitination by the SCF(FBXL17) complex takes place when aberrant BTB domain dimers are formed.; Acetylated at Lys-379 by EP300 which inhibits the interaction with NuRD complex and the transcriptional repressor function. Deacetylated by HDAC- and SIR2-dependent pathways.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4+ T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2.