PRODUCT CODE: ER0602

Bcl-2 Rabbit Polyclonal Antibody (ER0602)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Bcl-2 on different cell lysates using anti-Bcl-2 antibody at 1/500 dilution.
  • Western blot analysis of Bcl-2 on different cell lysates using anti-Bcl-2 antibody at 1/500 dilution.
  • ICC staining Bcl-2 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Bcl-2 rabbit polyclonal antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bcl-2 rabbit polyclonal antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Bcl-2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of Bcl-2 on different cell lysates using anti-Bcl-2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Bcl-2 Rabbit Polyclonal Antibody (ER0602)

Immunogen

Synthetic peptide within n-terminal human bcl-2.

Host

Rabbit

Positive Control

HL-60, Hela, HepG2, A431, human colon carcinoma tissue, human tonsil tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

26kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Bcl-2

SYNONYMS

Apoptosis regulator Bcl 2 antibody; Apoptosis regulator Bcl-2 antibody; Apoptosis regulator Bcl2 antibody; AW986256 antibody; B cell CLL/lymphoma 2 antibody; B cell leukemia/lymphoma 2 antibody; Bcl-2 antibody; Bcl2 antibody; BCL2_HUMAN antibody; C430015F12Rik antibody; D630044D05Rik antibody; D830018M01Rik antibody; Leukemia/lymphoma, B-cell, 2 antibody; Oncogene B-cell leukemia 2 antibody; PPP1R50 antibody; Protein phosphatase 1, regulatory subunit 50 antibody

SEQUENCE SIMILARITIES

Belongs to the Bcl-2 family.

TISSUE SPECIFICITY

Expressed in a variety of tissues.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

SUBCELLULAR LOCATION

Mitochondrion outer membrane, nucleus membrane

FUNCTION

Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments. Antibodies to Bcl-2 can be used with immunohistochemistry to identify cells containing the antigen. In healthy tissue, these antibodies will react with B-cells in the mantle zone, as well as some T-cells. However, there is a considerable increase in positive cells in follicular lymphoma, as well as many other forms of cancer. In some cases, the presence or absence of Bcl-2 staining in biopsies may be significant for the patient's prognosis or likelihood of relapse.

CITATIONS

  • Xie, Hongqing et al.

    Ethanolic extract of Cordyceps cicadae exerts antitumor effect on human gastric cancer SGC-7901 cells by inducing apoptosis, cell cycle arrest and endoplasmic reticulum stress. | Journal of Ethnopharmacology [2019]

  • Zhang, J. J., Song, C. G., ...

    Inhibition of mu-opioid receptor suppresses proliferation of hepatocellular carcinoma cells via CD147-p53-MAPK cascade signaling pathway. American journal of translational research, 13(5), 3967–3986.