PRODUCT CODE: ER0907

BAX Rabbit Polyclonal Antibody (ER0907)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/500 dilution.<br />
Positive control: <br />
  Lane 1: HepG2  Lane2 :Hela  Lane 3:Mouse embryonic stem cell  Lane 4:PC12  Lane 5:F9
  • Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/500 dilution.<br />
Positive control: <br />
  Lane 1: HepG2  Lane2 :Hela  Lane 3:Mouse embryonic stem cell  Lane 4:PC12  Lane 5:F9
  • ICC staining Bax in HepG2 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Bax in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Bax in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human gall bladder tissue using anti-Bax antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human cervical tissue using anti-Bax antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Bax antibody. Counter stained with hematoxylin.
Western blot analysis of Bax on different lysates using anti-Bax antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2 Lane2 :Hela Lane 3:Mouse embryonic stem cell Lane 4:PC12 Lane 5:F9

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

BAX Rabbit Polyclonal Antibody (ER0907)

Immunogen

Synthetic peptide within human bax aa 1-50 / 192.

Host

Rabbit

Positive Control

HepG2, Hela, mouse embryonic stem cell, PC12, F9 293, human gall bladder tissue, human cervical tissue, mouse testis tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

21 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

BAX

SYNONYMS

Apoptosis regulator BAX antibody; BAX antibody; Bax-protein antibody; BAX_HUMAN antibody; BAXA antibody; Baxdelta2G9 antibody; Baxdelta2G9omega antibody; Baxdelta2omega antibody; Bcl-2-like protein 4 antibody; BCL2 associated X protein antibody; BCL2 associated X protein omega antibody; BCL2 associated X protein transcript variant delta2 antibody; Bcl2-L-4 antibody; BCL2L4 antibody; membrane isoform alpha antibody

SEQUENCE SIMILARITIES

Belongs to the Bcl-2 family.

TISSUE SPECIFICITY

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

SUBCELLULAR LOCATION

Mitochondrion membrane, cytoplasm

FUNCTION

BAX is a member of the Bcl-2 gene family. Apoptosis regulator BAX promotes apoptosis by binding to and antagonizing the Bcl-2 protein. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX.

CITATIONS

  • Chen, Qiuhua et al.

    Sini decoction ameliorates sepsis-induced acute lung injury via regulating ACE2-Ang (1-7)-Mas axis and inhibiting the MAPK signaling pathway. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2019]

  • Metuzumab enhanced chemo......

    Metuzumab enhanced chemosensitivity and apoptosis in non-small cell lung carcinoma

  • Deficiency of CD147 Atte......

    Deficiency of CD147 Attenuated Non-alcoholic Steatohepatitis Progression in an NLRP3-Dependent Manner

  • Xie, Hongqing et al.

    Ethanolic extract of Cordyceps cicadae exerts antitumor effect on human gastric cancer SGC-7901 cells by inducing apoptosis, cell cycle arrest and endoplasmic reticulum stress. | Journal of Ethnopharmacology [2019]

  • Zhang, Yu et al.

    C21 steroid-enriched fraction refined from Marsdenia tenacissima inhibits hepatocellular carcinoma through the coordination of Hippo-Yap and PTEN-PI3K/AKT signaling pathways. | Oncotarget [2017]