PRODUCT CODE: ET1608-21

Bak Recombinant Rabbit Monoclonal Antibody [SU32-07] (ET1608-21)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

Western blot analysis of Bak on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: human skeletal muscle tissue lysate<br />
Lane 3: AGS cell lysate
  • Western blot analysis of Bak on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: human skeletal muscle tissue lysate<br />
Lane 3: AGS cell lysate
  • ICC staining of Bak in CRC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1608-21, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Bak antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-21, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Bak antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-21, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-Bak antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-21, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Bak was done on NIH/3T3 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1608-21, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Western blot analysis of Bak on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1608-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: human skeletal muscle tissue lysate
Lane 3: AGS cell lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Bak Recombinant Rabbit Monoclonal Antibody [SU32-07] (ET1608-21)

Immunogen

Synthetic peptide within human bak aa 1-50 / 211.

Host

Rabbit

Positive Control

Hela cell lysate, human skeletal muscle tissue lysate, AGS cell lysate, CRC, human lung tissue, mouse lung tissue, human stomach carcinoma tissue, NIH/3T3.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SU32-07

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

23 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Bak

SYNONYMS

Apoptosis regulator BAK antibody; BAK antibody; BAK like antibody; Bak NT antibody; BAK_HUMAN antibody; Bak1 antibody; Bcl 2 homologous antagonist/killer antibody; Bcl 2 like 7 protein antibody; Bcl-2 homologous antagonist/killer antibody; Bcl-2-like protein 7 antibody; BCL2 antagonist/killer 1 antibody; Bcl2 like 7 Protein antibody; Bcl2-L-7 antibody; BCL2L7 antibody; CDN1 antibody; Cell death inhibitor 1 antibody; MGC117255 antibody; MGC3887 antibody; NBak antibody; Pro apoptotic protein BAK antibody

SEQUENCE SIMILARITIES

Belongs to the Bcl-2 family.

TISSUE SPECIFICITY

Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.

SUBCELLULAR LOCATION

Mitochondrion membrane.

FUNCTION

The Bcl-2 family of proteins is characterized by its ability to modulate cell death (apoptosis) under a broad range of physiologic conditions. Bcl-2 and several related proteins function to inhibit apoptosis, while other members of the Bcl-2 family, such as Bax, accelerate death under various conditions. One member of the Bcl-2 family, designated Bak, functions primarily to enhance apoptotic cell death following appropriate activating signals and counteracts the protection from apoptosis provided by Bcl-2. Expression of Bak is widespread in a broad range of cells, including various long-lived, terminally differentiated cell types, suggesting that its cell-death-inducing activity is broadly distributed and that the regulation of inhibitors of apoptosis may represent an important determinant of tissue-specific modulation of apoptosis.