Bak Recombinant Rabbit Monoclonal Antibody [SU32-07] (ET1608-21)
Catalog# ET1608-21
Rabbit Monoclonal to Bak
-
WB
-
IF-Tissue
-
IHC-P
-
IP
-
Human
Lane 1: HeLa cell lysate
Lane 2: AGS cell lysate
Lane 3: HEK-293 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Bak Recombinant Rabbit Monoclonal Antibody [SU32-07] (ET1608-21)
Immunogen
Synthetic peptide within human bak aa 1-50 / 211.
Host
Rabbit
Positive Control
HeLa cell lysate, AGS cell lysate, HEK-293 cell lysate, human colon tissue, human stomach carcinoma tissue, human small intestine tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU32-07
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:500-1:1,000
-
IP
-
1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 23 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Bak
SYNONYMS
Apoptosis regulator BAK antibody;BAK antibody;BAK like antibody;Bak NT antibody;BAK_HUMAN antibody;Bak1 antibody;Bcl 2 homologous antagonist/killer antibody;Bcl 2 like 7 protein antibody;Bcl-2 homologous antagonist/killer antibody;Bcl-2-like protein 7 antibody;BCL2 antagonist/killer 1 antibody;Bcl2 like 7 Protein antibody;Bcl2-L-7 antibody;BCL2L7 antibody;CDN1 antibody;Cell death inhibitor 1 antibody;MGC117255 antibody;MGC3887 antibody;NBak antibody;Pro apoptotic protein BAK antibody
SEQUENCE SIMILARITIES
Belongs to the Bcl-2 family.
TISSUE SPECIFICITY
Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.
SUBCELLULAR LOCATION
Mitochondrion membrane.
FUNCTION
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.
CITATIONS
-
Wang Jinyu,et al
Schisandra chinensis lignans regulate and cooperate with endogenous cannabinoid systems to ameliorate intestinal barrier injury associated with depression
-
WB
-
Mouse
-
Citation
-
-
Chloroquine Intervenes Nephrotoxicity of Nilotinib through Deubiquitinase USP13‐Mediated Stabilization of Bcl‐XL
-
WB
-
Mouse
-
Citation
-
-
A network pharmacology approach and experimental validation to investigate the anticancer mechanism and potential active targets of ethanol extract of Wei-Tong-Xin against colorectal cancer through induction of apoptosis via PI3K/AKT signaling pathway
-
WB
-
Human
-
Citation
-
