PRODUCT CODE: ET7109-62

BAG2 Recombinant Rabbit Monoclonal Antibody [JE47-57] (ET7109-62)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

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Western blot analysis of BAG2 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of BAG2 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of BAG2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: A549 cell lysate<br />
Lane 1: K562 cell lysate<br />
Lane 2: HepG2 cell lysate
  • Flow cytometric analysis of BAG2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7109-62, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of BAG2 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-62, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

BAG2 Recombinant Rabbit Monoclonal Antibody [JE47-57] (ET7109-62)

Immunogen

Synthetic peptide within n-terminal human bag2.

Host

Rabbit

Positive Control

Jurkat cell lysates, Hela cell lysate, A549 cell lysate, K562 cell lysate, HepG2 cell lysate, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE47-57

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

BAG2

SYNONYMS

BAG 2 antibody; BAG family molecular chaperone regulator 2 antibody; BAG-2 antibody; BAG-family molecular chaperone regulator-2 antibody; Bag2 antibody; BAG2_HUMAN antibody; BCL 2 associated athanogene 2 antibody; Bcl-2-associated athanogene 2 antibody; BCL2 associated athanogene 2 antibody; BCL2-associated athanogene 2 antibody; dJ417I1.2 (BAG family molecular chaperone regulator 2) antibody; dJ417I1.2 antibody; KIAA0576 antibody; MGC149462 antibody; OTTHUMP00000016668 antibody

SUBCELLULAR LOCATION

Cytoskeleton, Cytosol.

FUNCTION

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. An increasing number of studies have indicated that BAG2 is involved in the pathogenesis of various diseases, including cancers and neurodegenerative diseases. BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.