PRODUCT CODE: EM1701-32

B-Raf Mouse Monoclonal Antibody [F1-F4-E10] (EM1701-32)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of B Raf on SiHa cell lysate using anti-B Raf antibody at 1/1,000 dilution.
  • Western blot analysis of B Raf on SiHa cell lysate using anti-B Raf antibody at 1/1,000 dilution.
  • ICC staining B Raf (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining B Raf (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-B Raf antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-B Raf antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-B Raf antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-B Raf antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-B Raf antibody. Counter stained with hematoxylin.
Western blot analysis of B Raf on SiHa cell lysate using anti-B Raf antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

B-Raf Mouse Monoclonal Antibody [F1-F4-E10] (EM1701-32)

Immunogen

Recombinant protein within human b-raf aa 49-239 / 766.

Host

Mouse

Positive Control

SiHa, LOVO, SH-SY5Y, rat testis tissue, human thyroid gland tissue, human colon cancer tissue, human placenta tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

F1-F4-E10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

84 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

B-Raf

SYNONYMS

FLJ95109 antibody; 94 kDa B raf protein antibody; B raf 1 antibody; B Raf proto oncogene serine threonine protein kinase antibody; B Raf proto oncogene, serine/threonine kinase antibody; B RAF1 antibody; B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody; BRAF 1 antibody; BRAF antibody; BRAF_HUMAN antibody; BRAF1 antibody; cRmil antibody; MGC126806 antibody; MGC138284 antibody; Murine sarcoma viral (v-raf) oncogene homolog B1 antibody; Murine sarcoma viral v raf oncogene homolog B1 antibody; NS7 antibody; Oncogen BRAF antibody; oncogene BRAF1 antibody; p94 antibody; Proto-oncogene B-Raf antibody; Proto-oncogene c-Rmil antibody; RAFB 1 antibody; RAFB1 antibody; RMIL antibody; Serine/threonine-protein kinase B-raf antibody; v raf murine sarcoma viral oncogene homolog B antibody; v raf murine sarcoma viral oncogene homolog B1 antibody; v-Raf murine sarcoma viral oncogene homolog B1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

TISSUE SPECIFICITY

Brain and testis.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-365 by SGK1 inhibits its activity.; Methylation at Arg-671 decreases stability and kinase activity.; Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Cell membrane.

FUNCTION

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.