B-Raf Mouse Monoclonal Antibody [F1-F4-E10] (EM1701-32)

UNIPROT #

P15056

PROTEIN NAME

B-Raf

SYNONYMS

FLJ95109 antibody;94 kDa B raf protein antibody;B raf 1 antibody;B Raf proto oncogene serine threonine protein kinase antibody;B Raf proto oncogene, serine/threonine kinase antibody;B RAF1 antibody;B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody;BRAF 1 antibody;BRAF antibody;BRAF_HUMAN antibody;BRAF1 antibody;cRmil antibody;MGC126806 antibody;MGC138284 antibody;Murine sarcoma viral (v-raf) oncogene homolog B1 antibody;Murine sarcoma viral v raf oncogene homolog B1 antibody;NS7 antibody;Oncogen BRAF antibody;oncogene BRAF1 antibody;p94 antibody;Proto-oncogene B-Raf antibody;Proto-oncogene c-Rmil antibody;RAFB 1 antibody;RAFB1 antibody;RMIL antibody;Serine/threonine-protein kinase B-raf antibody;v raf murine sarcoma viral oncogene homolog B antibody;v raf murine sarcoma viral oncogene homolog B1 antibody;v-Raf murine sarcoma viral oncogene homolog B1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

TISSUE SPECIFICITY

Brain and testis.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-365 by SGK1 inhibits its activity.; Methylation at Arg-671 decreases stability and kinase activity.; Ubiquitinated by RNF149; which leads to proteasomal degradation. Polyubiquitinated at Lys-578 in response to EGF.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm, Cell membrane.

FUNCTION

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.