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PRODUCT CODE: R1406-3

AXL Rabbit Polyclonal Antibody (R1406-3)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of AXL on different lysates using anti-AXL antibody at 1/100 dilution.<br /> Positive control:<br /> Lane 1: Mouse kidney tissue<br /> Lane 2: CRC
  • Western blot analysis of AXL on different lysates using anti-AXL antibody at 1/100 dilution.<br /> Positive control:<br /> Lane 1: Mouse kidney tissue<br /> Lane 2: CRC
  • Western blot analysis of AXL on human AXL recombinant protein using anti-AXL antibody at 1/1,000 dilution.
  • ICC staining AXL in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining AXL in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining AXL in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of AXL on different lysates using anti-AXL antibody at 1/100 dilution.
Positive control:
Lane 1: Mouse kidney tissue
Lane 2: CRC

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

AXL Rabbit Polyclonal Antibody (R1406-3)

Immunogen

Recombinant protein within human axl 1-200 aa.

Host

Rabbit

Positive Control

Mouse kidney tissue lysate, CRC cell lysate, human AXL recombinant protein lysate, Hela, LOVO, MCF-7.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

98 kDa(Predicted band)

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

P30530

PROTEIN NAME

AXL

SYNONYMS

Adhesion related kinase antibody; AI323647 antibody; Ark antibody; Axl antibody; AXL oncogene antibody; AXL receptor tyrosine kinase antibody; AXL transforming gene antibody; AXL transforming sequence/gene antibody; EC 2.7.10.1 antibody; JTK11 antibody; Oncogene AXL antibody; Tyro7 antibody; Tyrosine protein kinase receptor UFO antibody; Tyrosine-protein kinase receptor UFO antibody; UFO antibody; UFO_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

TISSUE SPECIFICITY

Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

POST-TRANSLATIONAL MODIFICATION

Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.; Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

The UFO family of receptor tyrosine kinases is comprised of subfamily members Rse (also designated Tyro3, Sky, Brt, Dtk, Etk2 and Tif), Axl (also designated UFO or ARK) and Mer (also designated Nyk or Eyk). Rse is expressed Preferentially in the adult brain with lower expression in other tissues. Axl is found at highest levels in heart and skeletal muscle. Mer has been identified as a tyrosine kinase is involved in the development of glioblastomas. It is expressed at highest levels in ovary, prostate , Lung and kidney. Gas6, a growth arrest specific gene, and the related anticoagulation factor Protein S, have been identified as ligands for the UFO family of receptors.