Axl Recombinant Rabbit Monoclonal Antibody [PSH06-68] (HA722740)
Catalog# HA722740
Rabbit Monoclonal to Axl
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Axl on different lysates with Rabbit anti-Axl antibody (HA722740) at 1/2,000 dilution.
Lane 1: NCI-H1299 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: HeLa cell lysate
Lane 4: HUVEC cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 98 kDa
Observed band size: 140 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722740) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Axl on different lysates with Rabbit anti-Axl antibody (HA722740) at 1/2,000 dilution.
Lane 1: NCI-H1299 cell lysate
Lane 2: Jurkat cell lysate (negative)
Lane 3: HeLa cell lysate
Lane 4: HUVEC cell lysate
Lane 5: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 98 kDa
Observed band size: 140 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722740) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.