Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00] (HA721919)
Catalog# HA721919
Rabbit Monoclonal to Axin1
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WB
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IHC-P
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Human
Lane 1: SW480 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: 293T cell lysate (10 µg/Lane)
Lane 4: K-562 cell lysate (10 µg/Lane)
Lane 5: Huh7 cell lysate (10 µg/Lane)
Predicted band size: 96 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721919) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Axin1 Recombinant Rabbit Monoclonal Antibody [PSH03-00] (HA721919)
Immunogen
Synthetic peptide within human axin1 aa 1-400 / 862.
Host
Rabbit
Positive Control
SW480 cell lysate, HeLa cell lysate, 293T cell lysate, K-562 cell lysate, Huh7 cell lysate, human colon cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH03-00
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:500-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 95.6 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Axin1
SYNONYMS
AI316800 antibody;AXIN antibody;Axin 1 antibody;Axin-1 antibody;axin1 antibody;AXIN1_HUMAN antibody;Axis inhibition protein 1 antibody;Axis Inhibitor 1 antibody;Fu antibody;fused antibody;Fused, mouse, homolog of antibody;hAxin antibody;Kb antibody;Ki antibody;kinky antibody;knobbly antibody;MGC132911 antibody;MGC52315 antibody;PPP1R49 antibody;Protein Fused antibody;Protein phosphatase 1 regulatory subunit 49 antibody
SUBCELLULAR LOCATION
Cytoplasm. Nucleus. Membrane. Cell membrane.
FUNCTION
Axin1 (Axis inhibition protein 1) and Axin2 are multidomain scaffold proteins that negatively regulate Wnt signaling. Axin1 interacts with APC, GSK-3β, Dvl, and β-catenin and promotes the GSK-3β-mediated phosphorylation and subsequent degradation of β-catenin . Upon stimulation of cells with Wnt, Axin1 is recruited to the membrane by phosphorylated LRP5/6, a process that is believed to be crucial for activation of Wnt signaling . In addition to its role in the Wnt signaling pathway, Axin1 forms a complex with MEKK1 and activates c-Jun amino-terminal kinase (JNK/SAPK) . Axin2 (also known as Conductin or Axil) can functionally substitute for Axin1 in mice . Axin2 itself is a direct target of the Wnt signaling pathway and therefore serves to control the duration and/or intensity of Wnt signaling through a negative feedback loop .
CITATIONS
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He Qiangqiang,et al
Smoking-induced CCNA2 expression promotes lung adenocarcinoma tumorigenesis by boosting AT2/AT2-like cell differentiation
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WB
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Human
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Citation
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