Stat3/Stat5 Signaling Antibody Sampler Kit (HAK21126)
Catalog# HAK21126
Reacts with
-
Human
Western blot analysis of Phospho-STAT3 (Y705) on different lysates with Rabbit anti-Phospho-STAT3 (Y705) antibody (ET1603-40) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Jurkat treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate
Lane 5: A431 cell lysate
Lane 6: A431 treated with 100ng/mL EGF for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 35 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-40) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Jurkat treated with 50ng/mL IFN alpha 1 for 30 minutes cell lysate
Lane 5: A431 cell lysate
Lane 6: A431 treated with 100ng/mL EGF for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 35 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-40) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.