PRODUCT CODE: 0908-2

Autoimmune regulator Rabbit Polyclonal Antibody (0908-2)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Mouse

Western blot analysis on 293 cell (A) transfected and (B) none transfected   with AIRE gene using anti-AIRE polyclonal antibody.
  • Western blot analysis on 293 cell (A) transfected and (B) none transfected   with AIRE gene using anti-AIRE polyclonal antibody.
  • Immunohistochemistry- staining AIRE in mouse thymus by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.
  • AIRE staining (red) in mouse embryonic stem cells (D3) by immunocytochemistry, nuclear staining with DAPI (blue) and SSEA-1 staining (green).
Western blot analysis on 293 cell (A) transfected and (B) none transfected with AIRE gene using anti-AIRE polyclonal antibody.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Autoimmune regulator Rabbit Polyclonal Antibody (0908-2)

Immunogen

Peptide

Host

Rabbit

Positive Control

Transfected 293 cells

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

59 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000

  • ICC

  • 1:500

  • IHC-P

  • 1:100

TARGET

UNIPROT #

PROTEIN NAME

Autoimmune regulator

SYNONYMS

AIRE antibody; AIRE_HUMAN antibody; AIRE1 antibody; APECED antibody; APECED protein antibody; APS1 antibody; APSI antibody; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein antibody; Autoimmune regulator antibody; Autoimmune regulator protein antibody; PGA1 antibody

TISSUE SPECIFICITY

Highly expressed in a few cells in the medulla of the thymus (medullary epithelial cells) (at protein level). Expressed in thymic but no peripheral B-cells. In secondary lymphoid organs, expressed in a discrete population of bone marrow-derived toleregenic antigen presenting cells (APCs) called extrathymic AIRE expressing cells (eTAC)(at protein level). Detected at very low levels in thymus, lymph node, liver, brain, ovary, lung, testis, kidney, heart, spleen, bone marrow, skeletal muscle and adrenal gland. Isoforms 1a to 1d predominate, isoforms 2a to 2d are intermediate and isoforms 3a to 3d are expressed at extremely low levels.

DEVELOPMENTAL STAGE

In the thymus, not expressed at 13.5 dpc but present at 16.5 dpc and postnatal day 1.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated.

SUBCELLULAR LOCATION

Cell nuclear, cytoplasm

FUNCTION

The Autoimmune Regulator (AIRE) is a human transcription factor expressed at higher level in thymus, pancreas, adrenal cortex, and testis. It is expressed at lower level in the spleen, fetal liver and lymph nodes. It contains zinc finger motifs and isoform 1 is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described. In the thymus it causes transcription of a wide selection of organ-specific genes which create proteins that are usually only expressed in peripheral tissues, creating an "immunological self-shadow" in the thymus. Defects in AIRE are a cause of autoimmune poly-endocrinopathy candidiasis ectodermal dystrophy (APECED), also known as autoimmune polyglandular syndrome type I (APS-1).