Aurora C Rabbit Polyclonal Antibody (ER1904-04)
Catalog# ER1904-04
Rabbit Polyclonal to Aurora C
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of Aurora C on different lysates with Rabbit anti-Aurora C antibody (ER1904-04) at 1/5,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: Huh7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Mouse testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 32 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1904-04) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT 116 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: Huh7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Mouse testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 32 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1904-04) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.