Positive control:
Lane 1: Hela
Lane 2: MCF-7
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
IP
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Aurora B Recombinant Rabbit Monoclonal Antibody [SC55-08] (ET1610-25)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
MCF-7, Hela, human tonsil tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC55-08
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
39 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Aurora B
SYNONYMS
AIK2 antibody; AIM-1 antibody; AIM1 antibody; ARK-2 antibody; ARK2 antibody; AurB antibody; AURKB antibody; AURKB_HUMAN antibody; Aurora 1 antibody; Aurora and Ipl1 like midbody associated protein 1 antibody; Aurora kinase B antibody; Aurora related kinase 2 antibody; Aurora- and Ipl1-like midbody-associated protein 1 antibody; Aurora-B antibody; Aurora-related kinase 2 antibody; Aurora/IPL1 related kinase 2 antibody; Aurora/IPL1-related kinase 2 antibody; IPL1 antibody; PPP1R48 antibody; Protein phosphatase 1 regulatory subunit 48 antibody; Serine/theronine kinase 12 antibody; Serine/threonine protein kinase 12 antibody; Serine/threonine-protein kinase 12 antibody; Serine/threonine-protein kinase aurora-B antibody; STK-1 antibody; STK1 antibody; STK12 antibody; STK5 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.
TISSUE SPECIFICITY
High level expression seen in the thymus. It is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.
POST-TRANSLATIONAL MODIFICATION
The phosphorylation of Thr-232 requires the binding to INCENP and occurs by means of an autophosphorylation mechanism. Thr-232 phosphorylation is indispensable for the AURKB kinase activity.; Ubiquitinated by different BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complexes. Ubiquitinated by the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, ubiquitination leads to removal from mitotic chromosomes and is required for cytokinesis. During anaphase, the BCR(KLHL21) E3 ubiquitin ligase complex recruits the CPC complex from chromosomes to the spindle midzone and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm, Chromosome.
FUNCTION
Aurora related kinase-1 (ARK-1, STK15, Aurora2, Aik1) and -2 (ARK-2, STK12, Aurora1) are centrosome-associated serine/ threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis. ARK-1 and -2 are expressed in the nucleus and localize to distinct portions of mitotic machinery such as the centrosome, spindle poles (ARK-1), and midbody (ARK-2) during mitosis. ARK-1 and -2 transcripts are present at high levels in human thymus and fetal liver. ARK-1 protein has elevated expression in colon carcinoma lines (HT-29, SNU-C2B, COLO 205, SW480, 837 and 948) and accumulates during metaphase in HeLa cells. ARK-2 protein levels are maximal during both S and G2/M phases, whereas ARK-1 protein is degraded after G2/M via the ubiquitin-proteasome pathway. ARK-2 has a unique genetic loci relative to ARK-1, suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression.