AU5 tag Rabbit Polyclonal Antibody (R1402-4)
Catalog# R1402-4
Rabbit Polyclonal to AU5 tag
Tested for
-
WB
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ELISA
Reacts with
-
Species independent
Western blot analysis of AU5 Tag on AU5 tagged fusion protein with Rabbit anti-AU5 Tag antibody (R1402-4) at 1/1,000 dilution.
Lane 1: AU5 tagged fusion protein, 1,000ng/Lane
Lane 2: AU5 tagged fusion protein, 200ng/Lane
Lane 3: AU5 tagged fusion protein, 50ng/Lane
Exposure time: 51 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1402-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: AU5 tagged fusion protein, 1,000ng/Lane
Lane 2: AU5 tagged fusion protein, 200ng/Lane
Lane 3: AU5 tagged fusion protein, 50ng/Lane
Exposure time: 51 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1402-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.