Western blot analysis of AU1 tag on AU1-tagged recombinant protein using anti-AU1 tag antibody at 1/2000 dilution.

Applications

WB
ELISA
IP
ICC

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

AU1 tag Mouse Monoclonal Antibody [F7-C2] (EM50803)

Immunogen

Peptide

Host

Mouse

Positive Control

AU1-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

F7-C2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein L purified.

Isotype

IgG2a

APPLICATION DILUTION

WB
1:2,000

TARGET

PROTEIN NAME

AU1 tag

SYNONYMS

AU1 epitope tag antibody; DTYRYI epitope tag antibody; DTYRYI tag antibody

FUNCTION

The recognized AU1 sequence represents the amino acid sequence DTYRYI, derived from the major capsid protein of bovine papillomavirus-1 (BPV-1). AU1 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. This antibody recognizes the AU1-tag fused to the carboxyl terminus of targeted proteins.