AU1 tag Mouse Monoclonal Antibody [F7-C2] (EM50803)

Western blot analysis of AU1 tag on AU1-tagged recombinant protein using anti-AU1 tag antibody at 1/2000 dilution.

Applications

WB
ELISA
IP
ICC

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

AU1 tag Mouse Monoclonal Antibody [F7-C2] (EM50803)

Immunogen

Peptide

Host

Mouse

Positive Control

AU1-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

F7-C2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein L purified.

Isotype

IgG2a

APPLICATION DILUTION

WB
1:2,000

TARGET

PROTEIN NAME

AU1 tag

SYNONYMS

AU1 epitope tag antibody; DTYRYI epitope tag antibody; DTYRYI tag antibody

FUNCTION

The recognized AU1 sequence represents the amino acid sequence DTYRYI, derived from the major capsid protein of bovine papillomavirus-1 (BPV-1). AU1 is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized by immunochemical methods. This antibody recognizes the AU1-tag fused to the carboxyl terminus of targeted proteins.

Protein FAM38A Mouse Monoclonal Antibody [2-10] (M1005-2)

Applications

WB
IHC-P
ICC

REACTIVITY

Human
Mouse

Western blot analysis of Protein FAM38A on recombinant protein using anti-Protein FAM38A antibody at 1/1000 dilution.

Caspase-1 Mouse Monoclonal Antibody [B0-6] (M1505-2)

Applications

WB
ICC
IHC-P

REACTIVITY

Human
Mouse
Rat

Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Caspase-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1505-2, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

PCSK9 Mouse Monoclonal Antibody [2F1] (EM1701-26)

Applications

WB
IHC-P
ICC
FC

REACTIVITY

Human
Mouse

Western blot analysis of PCSK9 on PANC-1 using anti-PCSK9 antibody at 1/100 dilution.

Goat IgG Light chain Mouse Monoclonal Antibody [B8-C4-G4] (M0910-2)

Applications

WB
ELISA
IP

REACTIVITY

Goat

Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Thymidine phosphorylase Mouse Monoclonal Antibody [A1A8] (HA600036)

Applications

REACTIVITY

Human

Western blot analysis of Thymidine phosphorylase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600036, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

MMAF Mouse Monoclonal Antibody [A5G11] (HA600035)

Applications

ELISA

REACTIVITY