ATPB Recombinant Rabbit Monoclonal Antibody [JM10-90] (ET1703-29)
Catalog# ET1703-29
Rabbit Monoclonal to ATPB
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
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Zebrafish
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: 293T cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-29) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ATPB Recombinant Rabbit Monoclonal Antibody [JM10-90] (ET1703-29)
Immunogen
Synthetic peptide within human atpb aa 141-184 / 529.
Host
Rabbit
Positive Control
Hela cell lysate, HepG2 cell lysate, 293T cell lysate, RH-35 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, zebrafish tissue lysates, 293T, A431, C6, human liver tissue, human kidney tissue, human uterus tissue, mouse liver tissue, mouse brain tissue, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM10-90
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:100-1:500
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IHC-P
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1:50-1:200
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IP
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1:10-1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 57 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ATPB
SYNONYMS
ATP 5B antibody;ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide antibody;ATP synthase subunit beta mitochondrial antibody;ATP synthase subunit beta, mitochondrial antibody;atp5b antibody;ATPB antibody;ATPB_HUMAN antibody;ATPMB antibody;ATPSB antibody;Epididymis secretory protein Li 271 antibody;HEL-S-271 antibody;Mitochondrial ATP synthase beta subunit antibody;Mitochondrial ATP Synthase Subunit Beta antibody;Mitochondrial ATP synthetase beta subunit antibody
SEQUENCE SIMILARITIES
Belongs to the ATPase alpha/beta chains family.
SUBCELLULAR LOCATION
Mitochondrion. Mitochondrion inner membrane. Peripheral membrane protein.
FUNCTION
ATP synthase F1 subunit beta, mitochondrial is an enzyme that in humans is encoded by the ATP5F1B gene. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
CITATIONS
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Yingfei Chen,et al
Exploring α-synuclein Interaction Partners and their Potential Clinical Implications for Parkinson's Disease
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WB
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Human
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Citation
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