ATP2A1 / SERCA1 Rabbit Polyclonal Antibody (ER1803-34)
Catalog# ER1803-34
Rabbit Polyclonal to ATP2A1
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
Western blot analysis of ATP2A1 / SERCA1 on different lysates with Rabbit anti-ATP2A1 / SERCA1 antibody (ER1803-34) at 1/2,000 dilution.
Lane 1: Mouse skeletal muscle tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate (no heat)
Lane 3: Mouse cerebellum tissue lysate (no heat) (negative)
Lane 4: Mouse heart tissue lysate (negative)
Lane 5: Rat skeletal muscle tissue lysate
Lane 6: Rat cerebellum tissue lysate (negative)
Lane 7: Rat heart tissue lysate (negative)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1803-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
ATP2A1 / SERCA1 Rabbit Polyclonal Antibody (ER1803-34)
Immunogen
Synthetis peptide within mouse atp2a1 aa 1-50 / 994.
Host
Rabbit
Positive Control
Mouse skeletal muscle tissue lysate, rat skeletal muscle tissue lysate, Human skeletal muscle tissue lysate, rat skeletal muscle tissue, human fetal skeletal muscle tissue, mouse skeletal muscle tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 110 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ATP2A1
SYNONYMS
fast twitch skeletal muscle isoform antibody;AT2A1_HUMAN antibody;ATP2A antibody;ATP2A1 antibody;ATPase Ca++ transporting cardiac muscle fast twitch 1 antibody;ATPase Ca++ transporting fast twitch 1 antibody;ATPase, Ca(2+)-transporting fast twitch 1 antibody;Calcium pump 1 antibody;Calcium transporting ATPase sarcoplasmic reticulum type fast twitch skeletal muscle isoform antibody;Calcium-transporting ATPase sarcoplasmic reticulum type antibody;EC 3.6.3.8 antibody;Endoplasmic reticulum class 1/2 Ca(2+) ATPase antibody;Fast skeletal muscle SR calcium ATPase antibody;OTTHUMP00000162561 antibody;OTTHUMP00000162562 antibody;Sarcoendoplasmic reticulum calcium ATPase antibody;Sarcoplasmic reticulum Ca(2+)-ATPase 1 antibody;Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 antibody;SERCA 1 antibody;SERCA1 antibody;SERCA1 truncated isoform, included antibody;SR Ca(2+) ATPase 1 antibody;SR Ca(2+)-ATPase 1 antibody
SEQUENCE SIMILARITIES
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
TISSUE SPECIFICITY
Skeletal muscle, fast twitch muscle (type II) fibers.
DEVELOPMENTAL STAGE
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
FUNCTION
Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
CITATIONS
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Caiqiao Zhang
Improvement of eggshell quality by dietary N-carbamylglutamate supplementation in laying chickens
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WB
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Chicken
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Citation
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