Lane 1: Wild-type A549 whole cell lysate (20 µg).
Lane 2: ATP citrate lyase knockout A549 whole cell lysate (20 µg).
ET1609-37 was shown to specifically react with ATP citrate lyase in wild-type A549 cells. No band was observed when ATP citrate lyase knockout sample was tested. Wild-type and ATP citrate lyase knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1609-37, 1/1,000) and Loading control antibody (Rabbit anti-HSP90, ET1605-56, 1/10,000)was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Recombinant Rabbit monoclonal primary
ATP citrate lyase Recombinant Rabbit Monoclonal Antibody [ST51-07] (ET1609-37)
Synthetic peptide within c-terminal human atp citrate lyase.
A549 cell lysate, CRC cell lysate, Hela cell lysate, Wild-type A549 whole cell lysates, Hela, A549, CRC, human thyroid tissue, human breast carcinoma tissue, mouse thyroid tissue, mouse kidney tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
Use at an assay dependent concentration.
ATP citrate lyase
ACL antibody; Acly antibody; ACLY_HUMAN antibody; ATP citrate (pro-S) lyase antibody; ATP citrate lyase antibody; ATP citrate synthase antibody; ATP-citrate (pro-S-)-lyase antibody; ATP-citrate synthase antibody; ATPcitrate synthase antibody; ATPCL antibody; Citrate cleavage enzyme antibody; CLATP antibody; OTTHUMP00000164773 antibody
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.; In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity. Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity). Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity).; ISGylated.; Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.; Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable).
ATP-citrate synthase, also designated ATP-citrate lyase or citrate cleavage enzyme, is a cytoplasmic homotetramer belonging to the succinate/malate CoA ligase family. The gene coding for this protein maps against chromosome 17q12-q21. ATP-citrate synthase catalyses the formation of acetyl-CoA and oxaloacetate from citrate and CoA. This product, Acetyl-CoA, is necessary for both fatty acid and cholesterol biosynthesis. ATP citrate-lyase is important in the biosynthesis of acetylcholine in nervous tissue.