PRODUCT CODE: ER1802-82

Atg12 Rabbit Polyclonal Antibody (ER1802-82)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of Atg12 on different lysates using anti-Atg12 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: A431<br />
      Lane 2: Human kidney tissue<br />
 Lane 3: 293T
  • Western blot analysis of Atg12 on different lysates using anti-Atg12 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: A431<br />
      Lane 2: Human kidney tissue<br />
 Lane 3: 293T
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Atg12 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 antibody. Counter stained with hematoxylin.
Western blot analysis of Atg12 on different lysates using anti-Atg12 antibody at 1/500 dilution.
Positive control:
Lane 1: A431
Lane 2: Human kidney tissue
Lane 3: 293T

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Atg12 Rabbit Polyclonal Antibody (ER1802-82)

Immunogen

Recombinant protein within human atg12 aa 1-120.

Host

Rabbit

Positive Control

A431, human kidney tissue lysate, 293T, human liver cancer tissue, human colon tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

8/15 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Atg12

SYNONYMS

APG12-like antibody; APG12L antibody; ATG12 antibody; ATG12 autophagy related 12 homolog (S. cerevisiae) antibody; ATG12 autophagy related 12 homolog antibody; ATG12_HUMAN antibody; Autophagy 12 antibody; Autophagy-related protein 12 antibody; FBR93 antibody; HAPG12 antibody; Ubiquitin-like protein ATG12 antibody

SEQUENCE SIMILARITIES

Belongs to the ATG12 family.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Acetylated by EP300.

SUBCELLULAR LOCATION

Cytoplasm. Membrane.

FUNCTION

Atg12 (autophagy-related protein 12), also known as APG12, APG12L, FBR93 or HAPG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the Atg12 family of proteins. Atg12 is a homolog of the yeast protein Apg12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. In yeast, autophagy requires a protein conjugation system consisting of Apg12 covalently bound at the carboxy terminal glycine to lysine 149 of Apg5. Similarly in humans, Atg12 is essential for autophagy and localizes to the cytoplasm where it is covalently bound to APG5, a conjugation reaction that requires APG7, Atg10 and ATP. The Atg12-APG5 conjugate functions as an important regulator of the autophagic process and is required for the change in membrane morphology and development of autophagosomes. Due to alternative splicing events, two Atg12 isoforms exist.