ATG10 Recombinant Rabbit Monoclonal Antibody [JE59-86] (HA721444)
Catalog# HA721444
Rabbit Monoclonal to ATG10
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of ATG10 on different lysates with Rabbit anti-ATG10 antibody (HA721444) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: K-562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: K-562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 30 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721444) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.