PRODUCT CODE: ER1706-34

ATF6 Rabbit Polyclonal Antibody (ER1706-34)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of ATF6 on Hela cell lysate using anti-ATF6 antibody at 1/500 dilution.
  • Western blot analysis of ATF6 on Hela cell lysate using anti-ATF6 antibody at 1/500 dilution.
  • ICC staining ATF6 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ATF6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of ATF6 on Hela cell lysate using anti-ATF6 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ATF6 Rabbit Polyclonal Antibody (ER1706-34)

Immunogen

Recombinant protein within human atf6 aa 168-392 / 670.

Host

Rabbit

Positive Control

Hela, A431, HUVEC, K562, rat brain tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze/thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

ATF6

SYNONYMS

Activating transcription factor 6 alpha antibody; Activating transcription factor 6 antibody; ATF 6 antibody; ATF6 alpha antibody; ATF6 antibody; ATF6-alpha antibody; ATF6A antibody; ATF6A_HUMAN antibody; cAMP dependent transcription factor ATF 6 alpha antibody; cAMP-dependent transcription factor ATF-6 alpha antibody; Cyclic AMP dependent transcription factor ATF 6 alpha antibody; DKFZp686P2194 antibody; ESTM49 antibody; FLJ21663 antibody; Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody; Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody

SEQUENCE SIMILARITIES

Belongs to the bZIP family. ATF subfamily.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.; N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).; Phosphorylated in vitro by MAPK14/P38MAPK.

SUBCELLULAR LOCATION

Endoplasmic reticulum membrane. Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. THBS4 promotes its nuclear shuttling.

FUNCTION

Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.

CITATIONS

  • Hao, Yan et al.

    2-Methylquinazoline derivative 23BB as a highly selective histone deacetylase 6 inhibitor alleviated cisplatin-induced acute kidney injury. | Bioscience Reports [2020]

  • Tan, Zhouke et al.

    Pharmacological and genetic inhibition of fatty acid-binding protein 4 alleviated cisplatin-induced acute kidney injury. | Journal of Cellular and Molecular Medicine [2019]

  • Huang, Zhuo et al.

    Activation of GPR120 by TUG891 ameliorated cisplatin-induced acute kidney injury via repressing ER stress and apoptosis. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2020]