Applications
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WB
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ICC
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IHC-P
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FC
REACTIVITY
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Human
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Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
ATF6 Rabbit Polyclonal Antibody (ER1706-34)
Immunogen
Recombinant protein within human atf6 aa 168-392 / 670.
Host
Rabbit
Positive Control
Hela, A431, HUVEC, K562, rat brain tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze/thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
75 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
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1:500-1:1,000
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ICC
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
ATF6
SYNONYMS
Activating transcription factor 6 alpha antibody; Activating transcription factor 6 antibody; ATF 6 antibody; ATF6 alpha antibody; ATF6 antibody; ATF6-alpha antibody; ATF6A antibody; ATF6A_HUMAN antibody; cAMP dependent transcription factor ATF 6 alpha antibody; cAMP-dependent transcription factor ATF-6 alpha antibody; Cyclic AMP dependent transcription factor ATF 6 alpha antibody; DKFZp686P2194 antibody; ESTM49 antibody; FLJ21663 antibody; Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody; Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody
SEQUENCE SIMILARITIES
Belongs to the bZIP family. ATF subfamily.
TISSUE SPECIFICITY
Ubiquitous.
POST-TRANSLATIONAL MODIFICATION
During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.; N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).; Phosphorylated in vitro by MAPK14/P38MAPK.
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane. Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. THBS4 promotes its nuclear shuttling.
FUNCTION
Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.
CITATIONS
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Tan, Zhouke et al.
Pharmacological and genetic inhibition of fatty acid-binding protein 4 alleviated cisplatin-induced acute kidney injury. | Journal of Cellular and Molecular Medicine [2019]
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WB
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IF
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mice
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Citation
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Hao, Yan et al.
2-Methylquinazoline derivative 23BB as a highly selective histone deacetylase 6 inhibitor alleviated cisplatin-induced acute kidney injury. | Bioscience Reports [2020]
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WB
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mice
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Citation
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Huang, Zhuo et al.
Activation of GPR120 by TUG891 ameliorated cisplatin-induced acute kidney injury via repressing ER stress and apoptosis. | Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie [2020]
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WB
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mice
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Citation
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