ATF6 Mouse Monoclonal Antibody [8D3] (EM1701-94)
Catalog# EM1701-94
Mouse Monoclonal to ATF6
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WB
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: RAW264.7 cell lysate (20 µg/Lane)
Lane 4: PC-12 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Mouse kidney tissue lysate (40 µg/Lane)
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-94) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
ATF6 Mouse Monoclonal Antibody [8D3] (EM1701-94)
Immunogen
Recombinant protein within human atf6 aa 1-670 / 670.
Host
Mouse
Positive Control
HeLa cell lysate, Jurkat cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, HeLa, mouse kidney tissue lysate, rat brain tissue, human placenta tissue, mouse testis tissue, HepG2.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
8D3
RRID
APPLICATION DILUTION
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WB
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1:1000-1:5,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 75 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
ATF6
SYNONYMS
Activating transcription factor 6 alpha antibody;Activating transcription factor 6 antibody;ATF 6 antibody;ATF6 alpha antibody;ATF6 antibody;ATF6-alpha antibody;ATF6A antibody;ATF6A_HUMAN antibody;cAMP dependent transcription factor ATF 6 alpha antibody;cAMP-dependent transcription factor ATF-6 alpha antibody;Cyclic AMP dependent transcription factor ATF 6 alpha antibody;DKFZp686P2194 antibody;ESTM49 antibody;FLJ21663 antibody;Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody;Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody
SEQUENCE SIMILARITIES
Belongs to the bZIP family. ATF subfamily.
TISSUE SPECIFICITY
Ubiquitous.
POST-TRANSLATIONAL MODIFICATION
During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.; N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).; Phosphorylated in vitro by MAPK14/P38MAPK.
SUBCELLULAR LOCATION
Endoplasmic reticulum. Nucleus.
FUNCTION
Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.
CITATIONS
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Tranexamic acid causes chondral injury through chondrocytes apoptosis induced by activating Endoplasmic Reticulum Stress
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WB
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Rat
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Citation
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DDIT3/CHOP mediates the inhibitory effect of ER stress on chondrocyte differentiation by AMPKα-SIRT1 pathway
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WB
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Mouse
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Citation
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Strontium Attenuates Hippocampal Damage via Suppressing Neuroinflammation in High-Fat Diet-Induced NAFLD Mice
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WB
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Mouse
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Citation
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Jinliang Nan,Yinuo Lin
Lipofectamine 2000/siRNA complexes cause endoplasmic reticulum unfolded protein response in human endothelial cells. Journal of cellular physiology, 234(11), 21166–21181.
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WB
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IF
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Human coronary artery endothelial cells (HCAECs)
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Citation
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ABCA1 deficiency-mediated glomerular cholesterol accumulation exacerbates glomerular endothelial injury and dysfunction in diabetic kidney disease
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IF
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Mouse
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Citation
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Liu W, Zhu M, Gong M, et al
Comparison of the Effects of Monounsaturated Fatty Acids and Polyunsaturated Fatty Acids on Liver Lipid Disorders in Obese Mice
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WB
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Mouse
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Citation
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PrPSc Inhibition and Cellular Protection of DBL on a Prion-Infected Cultured Cell via Multiple Pathways
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WB
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Mouse
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Citation
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Gankun Yuan,et al
Novel immunogenic cell death inducer combined with autophagy inhibitor to amplify photodynamic synergistic immunotherapy for triple-negative breast cancer
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WB
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Citation
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Heng Li,et al
Quantitative proteomics reveals the mechanism of endoplasmic reticulum stress-mediated pulmonary fibrosis in mice
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Oleic acid protects insulin-secreting INS-1E cells against palmitic acid-induced lipotoxicity along with an amelioration of ER stress
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WB
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Rat
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Citation
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Cao, X., Shu, Y., Chen, Y., Xu, Q., Guo, G., Wu, Z., Shao, M., Zhou, Y., Chen, M., Gong, Y., Li, C., Shi, Y., & Bu, H.
Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cellular and molecular gastroenterology and hepatology, S2352-345X(21)00072-2. Advance online publication.
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WB
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Mouse
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Citation
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Liu Wei,et al
Deactivation of the Unfolded Protein Response Aggravated Renal AA Amyloidosis in HSF1 Deficiency Mice
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WB
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Mouse
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Citation
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