PRODUCT CODE: EM1701-94

ATF6 Mouse Monoclonal Antibody [8D3] (EM1701-94)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of ATF6 on SiHa cell lysate using anti-ATF6 antibody at 1/1,000 dilution.
  • Western blot analysis of ATF6 on SiHa cell lysate using anti-ATF6 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATF6 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with ATF6 antibody at 1/50 dilution (pink purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of ATF6 on SiHa cell lysate using anti-ATF6 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

ATF6 Mouse Monoclonal Antibody [8D3] (EM1701-94)

Immunogen

Recombinant protein within human atf6 aa 1-670 / -670.

Host

Mouse

Positive Control

SiHa, rat brain tissue, human placenta tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

8D3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

ProG purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:100

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

ATF6

SYNONYMS

Activating transcription factor 6 alpha antibody; Activating transcription factor 6 antibody; ATF 6 antibody; ATF6 alpha antibody; ATF6 antibody; ATF6-alpha antibody; ATF6A antibody; ATF6A_HUMAN antibody; cAMP dependent transcription factor ATF 6 alpha antibody; cAMP-dependent transcription factor ATF-6 alpha antibody; Cyclic AMP dependent transcription factor ATF 6 alpha antibody; DKFZp686P2194 antibody; ESTM49 antibody; FLJ21663 antibody; Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody; Processed cyclic AMP-dependent transcription factor ATF-6 alpha antibody

SEQUENCE SIMILARITIES

Belongs to the bZIP family. ATF subfamily.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

During unfolded protein response, a fragment of approximately 50 kDa containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases.; N-glycosylated. The glycosylation status may serve as a sensor for ER homeostasis, resulting in ATF6 activation to trigger the unfolded protein response (UPR).; Phosphorylated in vitro by MAPK14/P38MAPK.

SUBCELLULAR LOCATION

Endoplasmic reticulum. Nucleus.

FUNCTION

Transmembrane glycoprotein of the endoplasmic reticulum that functions as a transcription activator and initiates the unfolded protein response (UPR) during endoplasmic reticulum stress. Cleaved upon ER stress, the N-terminal processed cyclic AMP-dependent transcription factor ATF-6 alpha translocates to the nucleus where it activates transcription of genes involved in the UPR. Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N9-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor. May play a role in foveal development and cone function in the retina.

CITATIONS

  • Li, Zhengzheng et al.

    Lipofectamine 2000/siRNA complexes cause endoplasmic reticulum unfolded protein response in human endothelial cells. | Journal of Cellular Physiology [2019]

    • WB

    • IF

    • Human coronary artery endothelial cells (HCAECs)

    • Citation

  • Cao, X., Shu, Y., Chen, Y.,...

    Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cellular and molecular gastroenterology and hepatology, S2352-345X(21)00072-2. Advance online publication.