ASS1 Mouse Monoclonal Antibody [11F1] (EM1701-87)
Catalog# EM1701-87
Mouse Monoclonal to ASS1
Tested for
-
WB
-
IHC-P
-
IF-Cell
-
FC
Reacts with
-
Human
-
Mouse
Knock-Out or Knock-Down validated
Western blot analysis of ASS1 on different lysates with Mouse anti-ASS1 antibody (EM1701-87) at 1/5,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: SiHa cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-87) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: SiHa cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-87) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.