Applications
-
WB
-
IHC-P
-
ICC
-
FC
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
ASS1 Mouse Monoclonal Antibody [11F1] (EM1701-87)
Immunogen
Recombinant protein within human ass1 aa 1-412 / 412.
Host
Mouse
Positive Control
HepG2, MCF-7, SiHa, human colon cancer tissue, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
11F1
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
47 kDa
Isotype
IgG2b
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
ICC
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
ASS1
SYNONYMS
Argininosuccinate synthase 1 antibody; Argininosuccinate synthase antibody; Argininosuccinate synthetase 1 antibody; ASS antibody; Ass-1 antibody; ass1 antibody; ASSA antibody; ASSY_HUMAN antibody; Citrulline aspartate ligase antibody; Citrulline--aspartate ligase antibody; CTLN1 antibody
SEQUENCE SIMILARITIES
Belongs to the argininosuccinate synthase family. Type 1 subfamily.
TISSUE SPECIFICITY
Expressed in adult liver.
DEVELOPMENTAL STAGE
Expressed in fetal liver and kidney.
POST-TRANSLATIONAL MODIFICATION
Acetylated by CLOCK in a circadian manner which negatively regulates its enzyme activity. Deacetylated by histone deacetylases.
SUBCELLULAR LOCATION
Cytosol.
FUNCTION
One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.