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PRODUCT CODE: EM1701-87

ASS1 Mouse Monoclonal Antibody [11F1] (EM1701-87)

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of ASS1 on HepG2 (1) and SiHa (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution.
  • Western blot analysis of ASS1 on HepG2 (1) and SiHa (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution.
  • ICC staining ASS1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ASS1 (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining ASS1 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASS1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASS1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with ASS1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of ASS1 on HepG2 (1) and SiHa (2) cell lysate using anti-ASS1 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

ASS1 Mouse Monoclonal Antibody [11F1] (EM1701-87)

Immunogen

Recombinant protein within human ass1 aa 1-412 / 412.

Host

Mouse

Positive Control

HepG2, MCF-7, SiHa, human colon cancer tissue, mouse testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

11F1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P00966

PROTEIN NAME

ASS1

SYNONYMS

Argininosuccinate synthase 1 antibody; Argininosuccinate synthase antibody; Argininosuccinate synthetase 1 antibody; ASS antibody; Ass-1 antibody; ass1 antibody; ASSA antibody; ASSY_HUMAN antibody; Citrulline aspartate ligase antibody; Citrulline--aspartate ligase antibody; CTLN1 antibody

SEQUENCE SIMILARITIES

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

TISSUE SPECIFICITY

Expressed in adult liver.

DEVELOPMENTAL STAGE

Expressed in fetal liver and kidney.

POST-TRANSLATIONAL MODIFICATION

Acetylated by CLOCK in a circadian manner which negatively regulates its enzyme activity. Deacetylated by histone deacetylases.

SUBCELLULAR LOCATION

Cytosol.

FUNCTION

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.