Positive control:
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Aspartate Aminotransferase Mouse Monoclonal Antibody [A2D7] (EM1901-50)
Immunogen
Recombinant protein within human got1 aa 16-211 / 413.
Host
Mouse
Positive Control
HepG2 cell lysate, K562 cell lysate, rat testis tissue, human liver tissue, human colon tissue, human colon carcinoma tissue, mouse kidney tissue, SW620.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A2D7
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G affinity purified.
MOLECULAR WEIGHT
46 kDa
Isotype
IgG2b
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Aspartate Aminotransferase
SYNONYMS
AATC_HUMAN antibody; Aspartate aminotransferase 1 antibody; Aspartate aminotransferase antibody; Aspartate aminotransferase cytoplasmic antibody; Aspartate aminotransferase cytosolic antibody; ASTQTL1 antibody; cAspAT antibody; cCAT antibody; cysteine aminotransferase, cytoplasmic antibody; cysteine transaminase, cytoplasmic antibody; cytoplasmic antibody; ec 2.6.1.1 antibody; GIG 18 antibody; GIG18 antibody; Glutamate oxaloacetate transaminase 1 antibody; Glutamate oxaloacetate transaminase soluble antibody; Glutamic oxaloacetic transaminase 1 soluble antibody; GOT 1 antibody; GOT1 antibody; Growth inhibiting protein 18 antibody; SGOT antibody; Transaminase A antibody
SEQUENCE SIMILARITIES
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.