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PRODUCT CODE: EM1901-51

Aspartate Aminotransferase Mouse Monoclonal Antibody [A2D6] (EM1901-51)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

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Western blot analysis of Aspartate Aminotransferase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: HepG2 cell lysate<br /> Lane 2: K562 cell lysate
  • Western blot analysis of Aspartate Aminotransferase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: HepG2 cell lysate<br /> Lane 2: K562 cell lysate
  • ICC staining of Aspartate Aminotransferase in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1901-51, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Flow cytometric analysis of Aspartate Aminotransferase was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (EM1901-51, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Aspartate Aminotransferase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Aspartate Aminotransferase Mouse Monoclonal Antibody [A2D6] (EM1901-51)

Immunogen

Recombinant protein within human got1 aa 16-211 / 413.

Host

Mouse

Positive Control

HepG2 cell lysate, K562 cell lysate, SiHa, SW620.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A2D6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G affinity purified.

MOLECULAR WEIGHT

46 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P17174

PROTEIN NAME

Aspartate Aminotransferase

SYNONYMS

AATC_HUMAN antibody; Aspartate aminotransferase 1 antibody; Aspartate aminotransferase antibody; Aspartate aminotransferase cytoplasmic antibody; Aspartate aminotransferase cytosolic antibody; ASTQTL1 antibody; cAspAT antibody; cCAT antibody; cysteine aminotransferase, cytoplasmic antibody; cysteine transaminase, cytoplasmic antibody; cytoplasmic antibody; ec 2.6.1.1 antibody; GIG 18 antibody; GIG18 antibody; Glutamate oxaloacetate transaminase 1 antibody; Glutamate oxaloacetate transaminase soluble antibody; Glutamic oxaloacetic transaminase 1 soluble antibody; GOT 1 antibody; GOT1 antibody; Growth inhibiting protein 18 antibody; SGOT antibody; Transaminase A antibody

SEQUENCE SIMILARITIES

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.